使用敲除细胞株进行验证

Anti-Topoisomerase II beta抗体(ab125297)

概述

  • 产品名称Anti-Topoisomerase II beta抗体
    参阅全部 Topoisomerase II beta 一抗
  • 描述
    兔多克隆抗体to Topoisomerase II beta
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Cow, Dog, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Topoisomerase II beta.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; K562; MCF7; HepG2. This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

性能

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应用

Our Abpromise guarantee covers the use of ab125297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa). Abcam recommends using milk as the blocking agent - 5%

靶标

  • 功能Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • 序列相似性Belongs to the type II topoisomerase family.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus > nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen MLAA 44 antibody
    • beta isozyme antibody
    • DNA topoisomerase 2 beta antibody
    • DNA topoisomerase 2-beta antibody
    • DNA topoisomerase II 180 kD antibody
    • DNA topoisomerase II antibody
    • DNA topoisomerase II beta antibody
    • DNA topoisomerase II beta isozyme antibody
    • Top 2 antibody
    • TOP 2B antibody
    • TOP IIB antibody
    • Top2 beta antibody
    • TOP2B antibody
    • TOP2B_HUMAN antibody
    • Top2beta antibody
    • TOPIIB antibody
    • Topo II beta antibody
    • Topoisomerase (DNA) II beta 180kDa antibody
    • Topoisomerase (DNA) II beta antibody
    • Topoisomerase IIb antibody
    • U937 associated antigen antibody
    see all

Anti-Topoisomerase II beta antibody 图像



  • Predicted band size : 183 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Topoisomerase II beta knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: NIH/3T3 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab125297 observed at 200 kDa. Red - loading control, ab7291, observed at 50 kDa.
    ab125297 was shown to recognize Topoisomerase II beta when Topoisomerase II beta knockout samples were used, along with additional cross-reactive bands. Wild-type and Topoisomerase II beta knockout samples were subjected to SDS-PAGE. ab125297 and ab7291 (loading control to alpha tubulin) were diluted 1 μg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-Topoisomerase II beta antibody (ab125297) at 1 µg/ml (Milk blocking - 5%)

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 183 kDa
    Observed band size : 183 kDa
    Additional bands at : 120 kDa,150 kDa,42 kDa,55 kDa,66 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    Abcam recommends using milk as the blocking agent - 5%. This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab125297 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-Topoisomerase II beta antibody (ab125297)参考文献

ab125297 has not yet been referenced specifically in any publications.

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