RabMAb

Anti-Topoisomerase II alpha (phospho T1343)抗体[EP1054Y] (ab52853)

概述

  • 产品名称Anti-Topoisomerase II alpha (phospho T1343)抗体[EP1054Y]
    参阅全部 Topoisomerase II alpha 一抗
  • 描述
    兔单克隆抗体[EP1054Y] to Topoisomerase II alpha (phospho T1343)
  • 特异性ab52853 only detects Topoisomerase II alpha phosphorylated on Threonine 1343.
  • 经测试应用适用于: IHC-P, WB, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr 1343 of human Topoisomerase II alpha.

  • 阳性对照
    • WB: HeLa cell lysates. IHC-P: Human cervical carcinoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab52853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 174 kDa (predicted molecular weight: 174 kDa).
ICC 1/50 - 1/100.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
    • 序列相似性Belongs to the type II topoisomerase family.
    • 翻译后修饰Phosphorylation has no effect on catalytic activity.
    • 细胞定位Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • alpha isozyme antibody
      • ATP hydrolyzing DNA topoisomerase II alfa antibody
      • DNA gyrase antibody
      • DNA topoisomerase (ATP hydrolyzing) antibody
      • DNA topoisomerase 2 alpha antibody
      • DNA topoisomerase 2-alpha antibody
      • DNA topoisomerase II 170 kD antibody
      • DNA topoisomerase II alpha isozyme antibody
      • DNA topoisomerase II antibody
      • DNA Topoisomerase2 antibody
      • TOP 2A antibody
      • TOP2 antibody
      • TOP2A antibody
      • TOP2A_HUMAN antibody
      • Topoisomerase DNA II alpha 170kDa antibody
      • TP2A antibody
      see all

    Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] 图像

    • All lanes : Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853) at 1/1000 dilution

      Lane 1 : HeLa cell lysate treated with AP
      Lane 2 : HeLa cell lysate untreated

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 174 kDa
      Observed band size : 174 kDa
    • ab52853 at 1/100 dilution staining Topoisomerase II alpha in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.

    Anti-Topoisomerase II alpha (phospho T1343) antibody [EP1054Y] (ab52853)参考文献

    ab52853 has not yet been referenced specifically in any publications.

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