Anti-Topoisomerase II alpha抗体(ab74715)

概述

  • 产品名称Anti-Topoisomerase II alpha抗体
    参阅全部 Topoisomerase II alpha 一抗
  • 描述
    兔多克隆抗体to Topoisomerase II alpha
  • 特异性ab74715 detects endogenous levels of total Topoisomerase II alpha protein.
  • 经测试应用适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Topoisomerase II alpha (N terminal). Immunogen is within the range of aa 1-50.
    Database link: P11388

  • 阳性对照
    • Human liver carcinoma tissue; extracts from HT-29 cells; A549 cells.

性能

应用

Our Abpromise guarantee covers the use of ab74715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Predicted molecular weight: 174 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/500.

靶标

  • 功能Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
  • 序列相似性Belongs to the type II topoisomerase family.
  • 翻译后修饰Phosphorylation has no effect on catalytic activity.
  • 细胞定位Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alpha isozyme antibody
    • ATP hydrolyzing DNA topoisomerase II alfa antibody
    • DNA gyrase antibody
    • DNA topoisomerase (ATP hydrolyzing) antibody
    • DNA topoisomerase 2 alpha antibody
    • DNA topoisomerase 2-alpha antibody
    • DNA topoisomerase II 170 kD antibody
    • DNA topoisomerase II alpha isozyme antibody
    • DNA topoisomerase II antibody
    • DNA Topoisomerase2 antibody
    • TOP 2A antibody
    • TOP2 antibody
    • TOP2A antibody
    • TOP2A_HUMAN antibody
    • Topoisomerase DNA II alpha 170kDa antibody
    • TP2A antibody
    see all

Anti-Topoisomerase II alpha antibody 图像

  • All lanes : Anti-Topoisomerase II alpha antibody (ab74715) at 1/500 dilution

    Lane 1 : extracts from HT-29 cells
    Lane 2 : extracts from HT-29 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 174 kDa
    Observed band size : 174 kDa
  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ab74715 at 1/50-1/100 dilution in the presence (+) or absence (-) of immunising peptide.
  • Immunofluorescence analysis of A549 cells, using ab74715 at 1/100-1/500 dilution in the presence (+) or absence (-) of immunising peptide.

Anti-Topoisomerase II alpha antibody (ab74715)参考文献

This product has been referenced in:
  • Gallego-Paez LM  et al. Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell 25:302-17 (2014). Human . Read more (PubMed: 24258023) »
  • Huang Y & Ratovitski EA Phosphorylated TP63 induces transcription of RPN13, leading to NOS2 protein degradation. J Biol Chem 285:41422-31 (2010). WB ; Human . Read more (PubMed: 20959455) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12 Bis tris)
Sample Human Cell lysate - whole cell (293T)
Specification 293T
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Jul 14 2014

Thank you for your inquiry. The antibodies (ab6078, ab8609, ab20034, ab22683, ab32575, ab66511, ab74715) need an MSDS only for the Sodium Azide in the storage buffer. For ab66511 there is no MSDS neccessary at all. The MSDS can be found on the da...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount 25 µg
Specification meiotic egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 28 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (X. laevis cell free egg extract)
Specification X. laevis cell free egg extract
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

提交于 Oct 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"