Anti-Topoisomerase II alpha抗体(ab12318)


  • 产品名称
    Anti-Topoisomerase II alpha抗体
    参阅全部 Topoisomerase II alpha 一抗
  • 描述
    兔多克隆抗体to Topoisomerase II alpha
  • 宿主
  • 经测试应用
    适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide representing a portion of human DNA topoisomerase II, alpha isozyme encoded within exon 35 (LocusLink ID 7153).

  • 阳性对照
    • HeLa cells. IHC-P: FFPE human testis tissue sections.
  • 常规说明
    Centrifuge tube to remove product from lid.



Our Abpromise guarantee covers the use of ab12318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/30000. Detects a band of approximately 178 kDa (predicted molecular weight: 184 kDa).
IP Use at 2-10 µg/mg of lysate.


  • 功能
    Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
  • 序列相似性
    Belongs to the type II topoisomerase family.
  • 翻译后修饰
    Phosphorylation has no effect on catalytic activity.
  • 细胞定位
    Cytoplasm. Nucleus > nucleoplasm. Generally located in the nucleoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alpha isozyme antibody
    • ATP hydrolyzing DNA topoisomerase II alfa antibody
    • DNA gyrase antibody
    • DNA topoisomerase (ATP hydrolyzing) antibody
    • DNA topoisomerase 2 alpha antibody
    • DNA topoisomerase 2-alpha antibody
    • DNA topoisomerase II 170 kD antibody
    • DNA topoisomerase II alpha isozyme antibody
    • DNA topoisomerase II antibody
    • DNA Topoisomerase2 antibody
    • TOP 2A antibody
    • TOP2 antibody
    • TOP2A antibody
    • TOP2A_HUMAN antibody
    • Topoisomerase DNA II alpha 170kDa antibody
    • TP2A antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryangeal squamous cell carcinoma labelling Topoisomerase II alpha with ab12318 at 1/1000 (1µg/ml). Detection: DAB.
  • IHC image of Topoisomerase II alpha staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12318, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Detection of Human Topo II Alpha by Western Blot and Immunoprecipitation.

    Samples: Whole cell lysate (25 mcg/lane for A; 0.5 mg for IP in B; 25 mcg for Input in B) from HeLa cells. Antibodies: Affinity purified rabbit anti-Topo II alpha antibody BL983 used at the indicated concentrations for WB (A) and IP (B). Control antibody (Ctrl. in A) and antibody for WB of immunoprecipitated Topo II alpha was a monoclonal antibody to Topo II alpha from another source. Detection: Chemiluminescence with exposure times of 5 minutes (A) or 30 seconds (B).


This product has been referenced in:
  • Schaefer-Klein JL  et al. Topoisomerase 2 Alpha Cooperates with Androgen Receptor to Contribute to Prostate Cancer Progression. PLoS One 10:e0142327 (2015). WB, ChIP ; Human . Read more (PubMed: 26560244) »
  • Yoshida H  et al. Brd4 bridges the transcriptional regulators, Aire and P-TEFb, to promote elongation of peripheral-tissue antigen transcripts in thymic stromal cells. Proc Natl Acad Sci U S A 112:E4448-57 (2015). WB . Read more (PubMed: 26216992) »

See all 7 Publications for this product


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Western blot
Mouse Cell lysate - whole cell (embryonic fibroblasts)
Loading amount
90 µg
embryonic fibroblasts
250 uM etoposide for 4h, 1 uM camptothecin for 1h
Gel Running Conditions
Reduced Denaturing (4-18% SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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提交于 Jul 07 2011