Anti-Topoisomerase I抗体[Mab1] (ab12407)

概述

  • 产品名称Anti-Topoisomerase I抗体[Mab1]
    参阅全部 Topoisomerase I 一抗
  • 描述
    小鼠单克隆抗体[Mab1] to Topoisomerase I
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (Human).

  • 阳性对照
    • Extract of A231 human kidney cells.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab12407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).

靶标

  • 功能The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
  • 疾病相关Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
  • 序列相似性Belongs to the eukaryotic type I topoisomerase family.
  • 翻译后修饰Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
  • 细胞定位Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA topoisomerase 1 antibody
    • DNA topoisomerase I antibody
    • NUP98 fusion gene antibody
    • TOP 1 antibody
    • TOP I antibody
    • TOP1 antibody
    • TOP1_HUMAN antibody
    • TOPI antibody
    • Topoisomerase (DNA) I antibody
    • Topoisomerase 1 antibody
    • Topoisomerase1 antibody
    • TopoisomeraseI antibody
    • Type I DNA topoisomerase antibody
    see all

Anti-Topoisomerase I antibody [Mab1] (ab12407)参考文献

ab12407 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and for providing more details of your protocol. This antibody has been tested for Western blot application using A231 human kidney cells. Thus, we would suggest running a positive control along with your samples. As w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"