Anti-Topoisomerase I抗体(ab93722)

概述

  • 产品名称Anti-Topoisomerase I抗体
    参阅全部 Topoisomerase I 一抗
  • 描述
    兔多克隆抗体to Topoisomerase I
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Topoisomerase I (internal sequence).

  • 阳性对照
    • Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab93722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

1/100 for 30 mins at RT. Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

靶标

  • 功能The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
  • 疾病相关Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
  • 序列相似性Belongs to the eukaryotic type I topoisomerase family.
  • 翻译后修饰Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
  • 细胞定位Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA topoisomerase 1 antibody
    • DNA topoisomerase I antibody
    • NUP98 fusion gene antibody
    • TOP 1 antibody
    • TOP I antibody
    • TOP1 antibody
    • TOP1_HUMAN antibody
    • TOPI antibody
    • Topoisomerase (DNA) I antibody
    • Topoisomerase 1 antibody
    • Topoisomerase1 antibody
    • TopoisomeraseI antibody
    • Type I DNA topoisomerase antibody
    see all

Anti-Topoisomerase I antibody 图像

  • ab93722 at 1/100 dilution staining Topoisomerase I in Human Breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

Anti-Topoisomerase I antibody (ab93722)参考文献

ab93722 has not yet been referenced specifically in any publications.

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