概述

  • 产品名称
    Anti-Topoisomerase I抗体
    参阅全部 Topoisomerase I 一抗
  • 描述
    兔多克隆抗体to Topoisomerase I
  • 特异性
    Ab28432 recognises Topoisomerase I.
  • 经测试应用
    适用于: WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Topoisomerase I protein (Human).

  • 阳性对照
    • HeLa nuclear extract.

性能

应用

Our Abpromise guarantee covers the use of ab28432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/5000. Predicted molecular weight: 91 kDa.
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/500.

靶标

  • 功能
    The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
  • 疾病相关
    Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
  • 序列相似性
    Belongs to the eukaryotic type I topoisomerase family.
  • 翻译后修饰
    Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
  • 细胞定位
    Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA topoisomerase 1 antibody
    • DNA topoisomerase I antibody
    • NUP98 fusion gene antibody
    • TOP 1 antibody
    • TOP I antibody
    • TOP1 antibody
    • TOP1_HUMAN antibody
    • TOPI antibody
    • Topoisomerase (DNA) I antibody
    • Topoisomerase 1 antibody
    • Topoisomerase1 antibody
    • TopoisomeraseI antibody
    • Type I DNA topoisomerase antibody
    see all

图片

  • ICC/IF image of ab28432 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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  • ICC/IF image of ab28432 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28432, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Jensen NF  et al. Characterization of DNA topoisomerase I in three SN-38 resistant human colon cancer cell lines reveals a new pair of resistance-associated mutations. J Exp Clin Cancer Res 35:56 (2016). Read more (PubMed: 27029323) »
  • Xia Q  et al. Characterization of the transcriptional machinery bound across the widely presumed type 2 diabetes causal variant, rs7903146, within TCF7L2. Eur J Hum Genet N/A:N/A (2014). Human . Read more (PubMed: 24667787) »

See all 9 Publications for this product

客户评价及客户问答

We have not tested ab28432 for mouse reactivity, but is likely that most topoisomerase antibodies, including ab28432, that react with human samples will react with mouse samples, given the 96% identity shared by the two proteins. This is based on align...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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