Anti-Topoisomerase I抗体[23B11] (ab200869)
Key features and details
- Mouse monoclonal [23B11] to Topoisomerase I
- Suitable for: WB
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG1
概述
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产品名称
Anti-Topoisomerase I抗体[23B11]
参阅全部 Topoisomerase I 一抗 -
描述
小鼠单克隆抗体[23B11] to Topoisomerase I -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Dog, Human
预测可用于: Xenopus laevis, Chinese hamster -
免疫原
Synthetic peptide corresponding to Human Topoisomerase I aa 650-750 conjugated to keyhole limpet haemocyanin.
Database link: P11387 -
阳性对照
- WB: Jurkat, MCF7, HEK293, NIH/3T3, NTERA-2, HeLa. Whole cell lysates of serum starved HeLa, HepG2, HEK293, SH-SY5Y, MDCK PC12, CMT 93, Neuro 2A and 3T3 tumor cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.09% Sodium azide
Constituent: PBS
With PEG and sucrose. -
Concentration information loading...
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纯度
Size exclusion -
克隆
单克隆 -
克隆编号
23B11 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab200869于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 91 kDa.
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说明 |
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WB
Use a concentration of 0.5 µg/ml. Predicted molecular weight: 91 kDa. |
靶标
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功能
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. -
疾病相关
Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. -
序列相似性
Belongs to the eukaryotic type I topoisomerase family. -
翻译后修饰
Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. -
细胞定位
Nucleus > nucleolus. Nucleus > nucleoplasm. Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli. - Information by UniProt
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数据库链接
- Entrez Gene: 7150 Human
- Entrez Gene: 21969 Mouse
- Entrez Gene: 64550 Rat
- Entrez Gene: 399263 Xenopus laevis
- Omim: 126420 Human
- SwissProt: P11387 Human
- SwissProt: Q04750 Mouse
- SwissProt: Q9WUL0 Rat
see all -
别名
- DNA topoisomerase 1 antibody
- DNA topoisomerase I antibody
- NUP98 fusion gene antibody
see all
图片
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All lanes : Anti-Topoisomerase I antibody [23B11] (ab200869) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 5 : NTERA-2 (Human malignant pluripotent embryonic carcinoma cell line) whole cell lysate
Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/2500 dilution
Predicted band size: 91 kDa
Observed band size: ~90 kDa why is the actual band size different from the predicted?The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate
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All lanes : Anti-Topoisomerase I antibody [23B11] (ab200869) at 0.5 µg/ml
Lane 1 : Whole cell lysate of serum starved HeLa cells
Lane 2 : Whole cell lysate of serum starved HepG2 cells
Lane 3 : Whole cell lysate of serum starved HEK293 cells
Lane 4 : Whole cell lysate of serum starved SH-SY5Y cells
Lane 5 : Whole cell lysate of serum starved MDCK cells
Lane 6 : Whole cell lysate of serum starved PC12 cells
Lane 7 : Whole cell lysate of serum starved CMT 93 cells
Lane 8 : Whole cell lysate of serum starved Neuro 2A cells
Lane 9 : Whole cell lysate of serum starved 3T3 tumor cells
Lysates/proteins at 20000 cells per lane.
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 30 secondsPVDF membrane.
数据表及文件
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Datasheet download
文献 (0)
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