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Synthetic peptide (Human) - which represents a portion of human Topoisomerase (DNA) II Binding Protein I encoded within exon 28.
Our Abpromise guarantee covers the use of ab2402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 0.5 - 2.5 µg/ml. May cross-react with other proteins such as CHK1 or possibly claspin.|
|WB||1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 173.3 kDa).|
|IHC-P||Use a concentration of 4 µg/ml.|
|ChIP||Use at an assay dependent concentration. PubMed: 17913746|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
Lane 1: WB Cell Lysate (5% of input IP)
Lane 2: IP control (normal rabbit IgG)
Lane 3: IP/WB
IP: Rabbit polyclonal to TopBP1 (1
WB: Rabbit polyclonal to TopBP1 (1/2000 dilution)
Positive control: HeLa Cells