Anti-TopBP1抗体(ab105109)
Key features and details
- Rabbit polyclonal to TopBP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TopBP1抗体
参阅全部 TopBP1 一抗 -
描述
兔多克隆抗体to TopBP1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Horse, Cow, Dog, Xenopus laevis, Macaque monkey, Gorilla, Chinese hamster -
免疫原
Synthetic peptide corresponding to Human TopBP1 aa 800-900 conjugated to keyhole limpet haemocyanin.
(Peptide available asab127877) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab105109于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 171 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 171 kDa). |
靶标
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功能
Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR. -
组织特异性
Highly expressed in heart, brain, placenta, lung and kidney. -
序列相似性
Contains 8 BRCT domains. -
翻译后修饰
Phosphorylated on serine and threonine residues in response to X-ray irradiation.
Ubiquitinated and degraded by the proteasome. X-ray irradiation reduces ubiquitination. -
细胞定位
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome. Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AFX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. - Information by UniProt
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数据库链接
- Entrez Gene: 11073 Human
- Entrez Gene: 235559 Mouse
- Omim: 607760 Human
- SwissProt: Q92547 Human
- SwissProt: Q6ZQF0 Mouse
- Unigene: 593379 Human
- Unigene: 259893 Mouse
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别名
- DNA topoisomerase 2 binding protein 1 antibody
- DNA topoisomerase 2-binding protein 1 antibody
- DNA topoisomerase II binding protein 1 antibody
see all
图片
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All lanes : Anti-TopBP1 antibody (ab105109) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 171 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 42 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute -
All lanes : Anti-TopBP1 antibody (ab105109) at 0.5 µg/ml
All lanes : Mouse ES whole cell lysate
Lysates/proteins at 200 µg per lane.
Secondary
All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 171 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 145 kDa (possible non-specific binding), 85 kDa (possible non-specific binding)
Exposure time: 30 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab105109 被引用在 2 文献中.
- Hirota T et al. SETDB1 Links the Meiotic DNA Damage Response to Sex Chromosome Silencing in Mice. Dev Cell 47:645-659.e6 (2018). PubMed: 30393076
- Vasileva A et al. The DNA damage checkpoint protein RAD9A is essential for male meiosis in the mouse. J Cell Sci 126:3927-38 (2013). PubMed: 23788429