RabMAb

Anti-TOP3B抗体[EP7779] - C-terminal (ab183520)

概述

  • 产品名称
    Anti-TOP3B抗体[EP7779] - C-terminal
    参阅全部 TOP3B 一抗
  • 描述
    兔单克隆抗体[EP7779] to TOP3B - C-terminal
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TOP3B aa 800 to the C-terminus. The exact sequence is proprietary.
    Database link: O95985
    (Peptide available as ab199552)

  • 阳性对照
    • K562, C6, Raw264.7, PC-12, NIH 3T3 and HepG2 cell lysates.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    EP7779
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab183520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human TOP3B peptide (ab199552).

靶标

  • 功能
    Possesses negatively supercoiled DNA relaxing activity.
  • 组织特异性
    Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.
  • 序列相似性
    Belongs to the prokaryotic type I/III topoisomerase family.
    Contains 1 Toprim domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA topoisomerase 3 beta 1 antibody
    • DNA topoisomerase 3-beta-1 antibody
    • DNA topoisomerase III beta 1 antibody
    • DNA topoisomerase III beta-1 antibody
    • FLJ39376 antibody
    • TOP 3B antibody
    • TOP3B antibody
    • TOP3B_HUMAN antibody
    • TOP3B1 antibody
    • Topo III beta antibody
    • Topoisomerase (DNA) III beta antibody
    • Topoisomerase DNA III beta antibody
    • Topoisomerase III beta antibody
    see all

图片

  • All lanes : Anti-TOP3B antibody [EP7779] - C-terminal (ab183520) at 1/2000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 97 kDa

  • All lanes :

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 97 kDa

实验方案

文献

ab183520 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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