概述
- 产品名称Anti-Tomosyn抗体
参阅全部 Tomosyn 一抗 - 描述兔多克隆抗体to Tomosyn
- 经测试应用适用于: WBmore details
- 种属反应性
预测可用于: Mouse, Rat, Human
- 免疫原
Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Tomosyn.
(Peptide available as ab45227.)
- 阳性对照
- This antibody gave a positive signal in the following tissues lysates: Rat Brain, Mouse Brain and Human Brain
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - 纯度Immunogen affinity purified
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab45228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa). |
靶标
- 相关性Tomosyn plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release by inhibiting the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane, via the displacement of munc18 from syntaxin-1. There are three splice variants of tomosyn designated m-tomosyn, b-tomosyn and s-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain.
- 细胞定位Cell Membrane and Cytoplasmic. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles
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数据库链接
- Entrez Gene: 134957 Human
- Entrez Gene: 78808 Mouse
- Entrez Gene: 81022 Rat
- Omim: 604586 Human
- SwissProt: Q5T5C0 Human
- SwissProt: Q8K400 Mouse
- SwissProt: Q9WU70 Rat
- Unigene: 93534 Human
see all -
别名
- FLJ30922 antibody
- Lethal(2) giant larvae protein homolog 3 antibody
- LGL3 antibody
see all
Anti-Tomosyn antibody 图像
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Western blot - Tomosyn antibody (ab45228)All lanes : Anti-Tomosyn antibody (ab45228) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 128 kDa
Observed band size : 128 kDa
Additional bands at : 30 kDa,52 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Anti-Tomosyn antibody (ab45228)参考文献
ab45228 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"