概述
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产品名称Anti-Tomosyn抗体
参阅全部 Tomosyn 一抗 -
描述兔多克隆抗体to Tomosyn
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宿主Rabbit
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经测试应用适用于: WBmore details
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种属反应性
预测可用于: Mouse, Rat, Human
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 850 - 950 of Human Tomosyn.
(Peptide available as ab45227.)
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阳性对照
- This antibody gave a positive signal in the following tissues lysates: Rat Brain, Mouse Brain and Human Brain
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab45228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa). |
靶标
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相关性Tomosyn plays a regulatory role in calcium-dependent exocytosis and neurotransmitter release by inhibiting the assembly of trans-SNARE complexes between transport vesicles and the plasma membrane, via the displacement of munc18 from syntaxin-1. There are three splice variants of tomosyn designated m-tomosyn, b-tomosyn and s-tomosyn. Although b-tomosyn is ubiquitously expressed, s-tomosyn and m-tomosyn are expressed primarily in brain.
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细胞定位Cell Membrane and Cytoplasmic. Note: Cytoplasmic, and associated with vesicular membranes and the plasma membrane. Detected at synapses and on synaptic vesicles
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数据库链接
- Entrez Gene: 134957 Human
- Entrez Gene: 78808 Mouse
- Entrez Gene: 81022 Rat
- Omim: 604586 Human
- SwissProt: Q5T5C0 Human
- SwissProt: Q8K400 Mouse
- SwissProt: Q9WU70 Rat
- Unigene: 93534 Human
see all -
别名
- FLJ30922 antibody
- Lethal(2) giant larvae protein homolog 3 antibody
- LGL3 antibody
see all
图片
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Western blot - Anti-Tomosyn antibody (ab45228)All lanes : Anti-Tomosyn antibody (ab45228) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 128 kDa
Observed band size: 128 kDa
Additional bands at: 30 kDa, 52 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
文献
ab45228 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"