概述

  • 产品名称Anti-TOMM22抗体
    参阅全部 TOMM22 一抗
  • 描述
    小鼠单克隆抗体to TOMM22
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-143 of Human TOMM22

性能

应用

Our Abpromise guarantee covers the use of ab57523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 15 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 细胞定位Mitochondrion outer membrane; Single-pass membrane protein
  • 数据库链接
  • 别名
    • 1C9 2 antibody
    • hTom 22 antibody
    • hTom22 antibody
    • Mitochondrial import receptor subunit TOM 22 homolog antibody
    • Mitochondrial import receptor subunit TOM22 homolog antibody
    • Mitochondrial import receptor Tom 22 antibody
    • Mitochondrial import receptor Tom22 antibody
    • MST 065 antibody
    • MST065 antibody
    • MSTP 065 antibody
    • MSTP065 antibody
    • TOM 22 antibody
    • TOM22 antibody
    • TOMM 22 antibody
    • Translocase of outer membrane 22 kDa subunit homolog antibody
    • Translocase of outer mitochondrial membrane 22 homolog antibody
    see all

Anti-TOMM22 antibody 图像

  • TOMM22 antibody (ab57523) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.


  • Predicted band size : 15 kDa
    TOMM22 antibody (ab57523) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.
  • ICC/IF image of ab57523 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57523, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab57523 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57523, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-TOMM22 antibody (ab57523)参考文献

This product has been referenced in:
  • Shi R  et al. BNIP3 Interacting with LC3 Triggers Excessive Mitophagy in Delayed Neuronal Death in Stroke. CNS Neurosci Ther N/A:N/A (2014). Mouse . Read more (PubMed: 25230377) »
  • Brinkmann CR  et al. Mitochondria and the lectin pathway of complement. J Biol Chem 288:8016-27 (2013). Flow Cyt ; Rat . Read more (PubMed: 23378531) »

See all 6 Publications for this product

Product Wall

Hello!

We also do recommend using RIPA buffer rather than NP40 for mitochondrial targets, would this be something you are able to do with your samples?

Thank you very much for your patience while I was in discussion with lab.

My colleague has confirmed that, they tested ab57523 with PC-12, Raw 264.7 and NIH/3T3 cells in western blot and they haven't observed any band at 88kDa. The band they o...

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Thank you for providing this extra information. The alignment shows a very low homology between the yeast protein and the human TOMM2 protein (www.uniprot.org/uniprot/Q9NS69), 16% only. This result suggests that unfortunately the TOMM2 ...

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Thank you very much for your inquiry. Unfortunately we do not have an antibody in our catalogue for the moment for which we can suggest a crossreactivity with the yeast SEC61 proteins. I am very sorry for this. Please see further below for the alignmen...

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