概述

  • 产品名称Anti-TOMM20抗体
    参阅全部 TOMM20 一抗
  • 描述
    小鼠单克隆抗体to TOMM20
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, IP, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Recombinant full length protein (GST-tag) corresponding to Human TOMM20 aa 1-145.
    Database link: Q15388

  • 阳性对照
    • WB: HeLa, PC-12 and NIH/3T3 cell lysates. IHC-P: Human small intestine. ICC/IF: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab56783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 16 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 10 µg/ml.

靶标

  • 功能Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
  • 序列相似性Belongs to the Tom20 family.
  • 细胞定位Mitochondrion outer membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • KIAA0016 antibody
    • MAS20 antibody
    • MGC117367 antibody
    • Mitochondrial 20 kDa outer membrane protein antibody
    • Mitochondrial import receptor subunit TOM20 homolog antibody
    • MOM19 antibody
    • Outer mitochondrial membrane receptor Tom20 antibody
    • TOM20 antibody
    • TOM20_HUMAN antibody
    • TOMM20 antibody
    • Translocase of outer mitochondrial membrane 20 homolog (yeast) antibody
    • Translocase of outer mitochondrial membrane 20 homolog type II antibody
    see all

Anti-TOMM20 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling TOMM20 with ab56783 at 3 µg/ml.

  • All lanes : Anti-TOMM20 antibody (ab56783)

    Lane 1 : HeLa cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate


    Predicted band size : 16 kDa
  • ab56783 staining TOMM20 in human keratinocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone and blocked with 1% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody (1/5000).

    See Abreview

  • ab56783 staining TOMM20 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with PBS + 0.1% Triton X-100 and blocked with PBS+ 0.5% BSA + 0.2% fish skin gelatin for 1 hour at 25°C. Samples were incubated with primary antibody (1/2000 in PBS + 1% BSA) for 24 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody (1/500).

    See Abreview

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling TOMM20 with ab56783 at 10 µg/ml.

  • TOMM20 was immunoprecipitated using 0.5 mg HepG2 whole cell extract, 5 µg of Mouse monoclonal to TOMM20 (ab56783) and 50 µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10 min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation.
    Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 min at 70°C; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56783.
    Secondary: Protein G-HRP at 1/500 dilution.
    Band: 14kDa: TOMM20. Non specific - 25kDa: We are unsure as to the identity of this extra band.

  • Overlay histogram showing HeLa cells stained with ab56783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56783, 1 μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was a mix of mouse IgG1 [ICIGG1], (ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4%PFA/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-TOMM20 antibody (ab56783)参考文献

This product has been referenced in:
  • Branco AF  et al. Gene Expression Profiling of H9c2 Myoblast Differentiation towards a Cardiac-Like Phenotype. PLoS One 10:e0129303 (2015). Read more (PubMed: 26121149) »
  • Yuan Y  et al. Correlative light and electron microscopic analyses of mitochondrial distribution in blastomeres of early fish embryos. FASEB J 28:577-85 (2014). Read more (PubMed: 24136588) »

See all 13 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Sample Fruit fly (Drosophila melanogaster) Cell (heart tube myocyte)
Specification heart tube myocyte
Permeabilization Yes - 1% triton x-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 11 2013

Thank you for contacting us.



We searched information of TOMM20 protein in database, and according to the description on the database, TOMM20 is a mitochondrion outer membrane.(http://www.uniprot.org/uniprot/Q15388). As such this ...

Read More
Application Western blot
Sample Mouse Cell lysate - other (Liver Mitochondrial fraction)
Loading amount 20 µg
Specification Liver Mitochondrial fraction
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 May 15 2012

Thank you for contacting us. Anti-TOMM20 antibody (ab56783) has been protein G purified and is supplied in PBS at pH 7.2. I can therefore confirm that the antibody supplied does not contain any other carrier proteins or gelatine in the vial. I ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human keratinocytes)
Specification human keratinocytes
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 May 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (keratinocytes)
Loading amount 80 µg
Specification keratinocytes
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Apr 21 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Hippocampal culture)
Specification Hippocampal culture
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Sep 01 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.1% Triton X-100/PBS
Specification HeLa
Blocking step 0.5% BSA, 0.2% fish skin gelatin/PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 28 2010

Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount 25 µg
Specification meiotic egg extract
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Loading amount 10 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (10% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

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提交于 Jan 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"