概述

  • 产品名称
  • 描述
    兔多克隆抗体to TOM1L2
  • 经测试应用
    适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 394-457 (

    SLAEQRKTVTYEDPQAVGGLASALDNRKQSSEGIPVAQPSVMDDIEVWLR TDLKGDDLEEGVTS

    ) of Human TOM1L2.

  • 阳性对照
    • A549, HeLa, Jurkat and NT2D1 cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 56 kDa.
IP Use a concentration of 5 µg/ml.

靶标

  • 功能
    Probable role in protein transport. May regulate growth factor-induced mitogenic signaling.
  • 组织特异性
    Ubiquitously expressed with higher expression in heart and skeletal muscle.
  • 序列相似性
    Belongs to the TOM1 family.
    Contains 1 GAT domain.
    Contains 1 VHS domain.
  • 结构域
    The GAT domain mediates interaction with TOLLIP.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ32746 antibody
    • Srebf1 antibody
    • Target of Myb-like protein 2 antibody
    • Target of myb1 like 2 antibody
    • Target of myb1-like 2 (chicken) antibody
    • TM1L2_HUMAN antibody
    • TOM1 antibody
    • TOM1-like 2 antibody
    • TOM1-like protein 2 antibody
    • TOM1L 2 antibody
    • Tom1l2 antibody
    see all

图片

  • TOM1L2 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to TOM1L2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96320.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)

    >Band: 56kDa; TOM1L2

  • All lanes : Anti-TOM1L2 antibody (ab96320) at 1/1000 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : NT2D1 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 56 kDa
    7.5% SDS PAGE

文献

This product has been referenced in:
  • Masters TA  et al. MYO6 Regulates Spatial Organization of Signaling Endosomes Driving AKT Activation and Actin Dynamics. Cell Rep 19:2088-2101 (2017). Read more (PubMed: 28591580) »
  • Arden SD  et al. Loss of cargo binding in the human myosin VI deafness mutant (R1166X) leads to increased actin filament binding. Biochem J 473:3307-19 (2016). WB . Read more (PubMed: 27474411) »

See all 6 Publications for this product

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