概述

  • 产品名称Anti-TOM1抗体
    参阅全部 TOM1 一抗
  • 描述
    兔多克隆抗体to TOM1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 450-492 of Human TOM1 (NP_005479.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab99356 was affinity purified using an epitope specific to TOM1 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab99356 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性TOM1 may be involved in intracellular trafficking. In conjunction with Tollip it plays an important role in recruiting clathrin onto endosomes and may modulate endosomal functions.
  • 细胞定位Cytoplasm. Membrane; Peripheral membrane protein
  • 数据库链接
  • 别名
    • FLJ33404 antibody
    • OTTHUMP00000028777 antibody
    • Target of myb 1 antibody
    • Target of Myb protein 1 antibody
    • Target of myb1 antibody
    • TOM 1 antibody
    see all

Anti-TOM1 antibody 图像

  • All lanes : Anti-TOM1 antibody (ab99356) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 54 kDa


    Exposure time : 30 seconds
  • ab99356, at 1 µg/ml, detecting TOM1 in HeLa whole cell lysate by Western blot analysis following Immunoprecipitation (IP). Detection utilized ECL with a 3 second exposure.
    For immunoprecipitation, ab99356 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99355 (a rabbit anti-TOM1 antibody which recognizes an upstream epitope).
    Lane 2; IP using ab99356.
    Lane 3; IP using control IgG.

Anti-TOM1 antibody (ab99356)参考文献

ab99356 has not yet been referenced specifically in any publications.

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