概述

  • 产品名称Anti-TOM1抗体
    参阅全部 TOM1 一抗
  • 描述
    小鼠单克隆抗体to TOM1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Recombinant fragment: GLAGALDARQ QSTGAIPVTQ ACLMEDIEQW LSTDVGNDAE EPKGVTSEEF DKFLEERAKA ADRLPNLSSP SAEGPPGPPS GPAPRKKTQE KDDDMLFA, corresponding to amino acids 394-491 of Human TOM1 (NP_005479) with a proprietory tag.

  • 阳性对照
    • HeLa cell lysate. Recombinant tagged human TOM1 fragment.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab77649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged TOM1 is approximately 30ng/ml when used as a capture antibody.

靶标

Anti-TOM1 antibody 图像

  • Anti-TOM1 antibody (ab77649) at 1 µg/ml + Recombinant tagged human TOM1 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 54 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-TOM1 antibody (ab77649) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 54 kDa
    Observed band size : 54 kDa

Anti-TOM1 antibody (ab77649)参考文献

ab77649 has not yet been referenced specifically in any publications.

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