概述

  • 产品名称Anti-Tollip抗体
    参阅全部 Tollip 一抗
  • 描述
    兔多克隆抗体to Tollip
  • 经测试应用适用于: IHC-P, ELISA, WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to 16 amino acids near to the C terminus of the human Tollip protein.

  • 阳性对照
    • Rat brain cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab37155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 2 µg/ml.
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
ICC Use a concentration of 2 µg/ml.

靶标

Anti-Tollip antibody 图像

  • Lane 1 : Anti-Tollip antibody (ab37155) at 1 µg/ml
    Lane 2 : Anti-Tollip antibody (ab37155) at 2 µg/ml

    Lane 1 : Rat brain cell lysate
    Lane 2 : Rat brain cell lysate


    Predicted band size : 30 kDa
    Observed band size : 30 kDa
  • ab37155 at 2 µg/ml staining Tollip in THP-1 cells by ICC. Secondary antibody was biotin conjugated donkey anti-rabbit.
  • ab37155 staining Tollip in human placenta.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Tollip antibody (ab37155)参考文献

ab37155 has not yet been referenced specifically in any publications.

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