Anti-TOB (phospho S164)抗体(ab78915)


  • 产品名称Anti-TOB (phospho S164)抗体
    参阅全部 TOB 一抗
  • 描述
    兔多克隆抗体to TOB (phospho S164)
  • 特异性ab78915 detects endogenous levels of TOB only when phosphorylated at serine 164.
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human TOB around the phosphorylation site of serine 164 (A-V-SP-P-T).

  • 阳性对照
    • Human brain tissue; HT-29 cell extracts, treated with serum (20%, 15mins).



Our Abpromise guarantee covers the use of ab78915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
IHC-P 1/50 - 1/100.


  • 相关性The Tob/Btg family of proteins consists of a large number of members. These proteins have a common domain in their amino terminal end and may have anti-proliferative activity in various cell types. The Tob protein was identified in a search for molecules that interact with the receptor tyrosine kinase ErbB2. Active ErbB2 has a negative effect on the anti-proliferative activity of Tob. However, Tob is phosphorylated on serine and threonine residues but not on tyrosine, suggesting that active ErbB2 activates a Ser/Thr kinase that phosphorylates Tob. Unphosphorylated Tob suppresses expression of cyclin D1. It was shown that active p90Rsk1 kinase (known to be activated by protein-tyrosine kinase receptor) phosphorylates Tob on serine and threonine residues in vitro. In addition, Erk1/Erk2 MAP kinases phosphorylate Tob in vivo and in vitro, resulting in suppression of the anti-proliferative activity of Tob. Homozygous Tob knockout mice develop greater bone mass resulting from increased numbers of osteoblasts. Furthermore, it has been shown in osteoblasts, that upon BMP2 (bone morphogenetic protein) activation, Tob associates with receptor regulated Smads (Smad 1, 5, and 8). Thus, osteoblast proliferation and differentiation is negatively regulated by Tob protein through the Smad proteins.
  • 数据库链接
  • 别名
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Anti-TOB (phospho S164) antibody 图像

  • ab78915 at 1/50 dilution staining TOB in human brain by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.
  • All lanes : Anti-TOB (phospho S164) antibody (ab78915) at 1/500 dilution

    Lane 1 : HT-29 cell extracts, treated with serum (20%, 15mins)
    Lane 2 : HT-29 cell extracts, treated with serum (20%, 15mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa

Anti-TOB (phospho S164) antibody (ab78915)参考文献

ab78915 has not yet been referenced specifically in any publications.

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