概述

  • 产品名称Anti-TOB抗体
    参阅全部 TOB 一抗
  • 描述
    兔多克隆抗体to TOB
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Chicken, Cow, Dog, Human, Pig
  • 免疫原

    A region within synthetic peptide: TFAATKFGST KMKNSGRSSK VARTSPINLG LTVNVNDLLK QKAISSSVHS, corresponding to internal sequence amino acids 181-230 of Human TOB

  • 阳性对照
    • NIH 3T3 lysate

性能

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应用

Our Abpromise guarantee covers the use of ab48826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/62500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性The Tob/Btg family of proteins consists of a large number of members. These proteins have a common domain in their amino terminal end and may have anti-proliferative activity in various cell types. The Tob protein was identified in a search for molecules that interact with the receptor tyrosine kinase ErbB2. Active ErbB2 has a negative effect on the anti-proliferative activity of Tob. However, Tob is phosphorylated on serine and threonine residues but not on tyrosine, suggesting that active ErbB2 activates a Ser/Thr kinase that phosphorylates Tob. Unphosphorylated Tob suppresses expression of cyclin D1. It was shown that active p90Rsk1 kinase (known to be activated by protein-tyrosine kinase receptor) phosphorylates Tob on serine and threonine residues in vitro. In addition, Erk1/Erk2 MAP kinases phosphorylate Tob in vivo and in vitro, resulting in suppression of the anti-proliferative activity of Tob. Homozygous Tob knockout mice develop greater bone mass resulting from increased numbers of osteoblasts. Furthermore, it has been shown in osteoblasts, that upon BMP2 (bone morphogenetic protein) activation, Tob associates with receptor regulated Smads (Smad 1, 5, and 8). Thus, osteoblast proliferation and differentiation is negatively regulated by Tob protein through the Smad proteins.
  • 数据库链接
  • 别名
    • APRO 6 antibody
    • APRO6 antibody
    • MGC104792 antibody
    • MGC34446 antibody
    • PIG 49 antibody
    • PIG49 antibody
    • Proliferation inducing gene 49 antibody
    • Protein Tob1 antibody
    • RP23 244C22.1 antibody
    • TOB 1 antibody
    • Tob 1 protein antibody
    • TOB antibody
    • TOB1 antibody
    • Tob1 protein antibody
    • Transducer of ERBB 2 1 antibody
    • Transducer of erbB 2 antibody
    • Transducer of ERBB2 1 antibody
    • Transducer of erbB2 antibody
    • Trob antibody
    • TROB 1 antibody
    • TROB1 antibody
    see all

Anti-TOB antibody 图像

Anti-TOB antibody (ab48826)参考文献

ab48826 has not yet been referenced specifically in any publications.

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