概述

  • 产品名称Anti-TNR抗体
    参阅全部 TNR 一抗
  • 描述
    兔多克隆抗体to TNR
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen: QGLQFKTITE TTVEVQWEPF SFSFDGWEIS FIPKNNEGGV IAQVPSDVTS FNQTGLKPGE EYIVNVVALK, corresponding to amino acids 419-488 of Human TNR.

  • 阳性对照
    • U 251MG cells; Human hippocampus tissue.

性能

应用

Our Abpromise guarantee covers the use of ab121916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
IHC-P 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance.
  • 组织特异性Brain specific.
  • 序列相似性Belongs to the tenascin family.
    Contains 5 EGF-like domains.
    Contains 1 fibrinogen C-terminal domain.
    Contains 9 fibronectin type-III domains.
  • 结构域The EGF-like domains mediate interaction with CNTN1. The fibronectin type-III domains 3-5 mediate interaction with BCAN. The fibronectin type-III domains 1-2 and 7-9 mediate interaction with SCN2B.
  • 翻译后修饰Contains N-linked oligosaccharides, O-linked sialylated structures and O-linked chondroitin sulfate glycosaminoglycans. Contains N-linked oligosaccharides with a sulfated carbohydrate structure (By similarity). O-glycosylated on Thr-36 or Thr-37 with a core 1 or possibly core 8 glycan.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Janusin antibody
    • MGC149328 antibody
    • Restrictin antibody
    • Tenascin R (restrictin, janusin) antibody
    • Tenascin-R antibody
    • TENR_HUMAN antibody
    • TN-R antibody
    • Tnr antibody
    see all

Anti-TNR antibody 图像

  • ab121916, at 1/600 dilution, staining TNR in paraffin embedded Human hippocampus tissue by Immunohistochemistry.
  • ab121916, at 4 µg/ml, staining TNR in PFA/Triton X-100 fixed/permeabilized U 251MG cells by Immunofluorescence (green).

Anti-TNR antibody (ab121916)参考文献

ab121916 has not yet been referenced specifically in any publications.

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