重组Anti-TNPO3抗体[EPR5264] (ab109386)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5264] to TNPO3
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TNPO3抗体[EPR5264]
参阅全部 TNPO3 一抗 -
描述
兔单克隆抗体[EPR5264] to TNPO3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, Jurkat, Ramos and HepG2 whole cell lysate (ab7900); Human kidney tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 4.70 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5264 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109386于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 104 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 104 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Seems to function in nuclear protein import as nuclear transport receptor. In vitro, mediates the nuclear import of splicing factor SR proteins SFRS1 and SFRS2, by recognizing phosphorylated RS domains. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 23534 Human
- Entrez Gene: 320938 Mouse
- Entrez Gene: 296954 Rat
- Omim: 610032 Human
- SwissProt: Q9Y5L0 Human
- SwissProt: Q6P2B1 Mouse
- Unigene: 193613 Human
- Unigene: 276381 Mouse
see all -
别名
- TNPO 3 antibody
- Imp12 antibody
- Importin 12 antibody
see all
图片
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All lanes : Anti-TNPO3 antibody [EPR5264] (ab109386) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 104 kDa -
ab109386, at 1/100 dilution, staining TNPO3 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab109386 被引用在 6 文献中.
- Marco S et al. Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion. Nat Commun 12:6829 (2021). PubMed: 34819513
- Bochnakian A et al. Interferon-Inducible MicroRNA miR-128 Modulates HIV-1 Replication by Targeting TNPO3 mRNA. J Virol 93:N/A (2019). PubMed: 31341054
- Vihola A et al. Novel mutation in TNPO3 causes congenital limb-girdle myopathy with slow progression. Neurol Genet 5:e337 (2019). PubMed: 31192305
- Mallery DL et al. Cellular IP6 Levels Limit HIV Production while Viruses that Cannot Efficiently Package IP6 Are Attenuated for Infection and Replication. Cell Rep 29:3983-3996.e4 (2019). PubMed: 31851928
- Meehan AM et al. A cyclophilin homology domain-independent role for Nup358 in HIV-1 infection. PLoS Pathog 10:e1003969 (2014). WB . PubMed: 24586169
- Torella A et al. Next-generation sequencing identifies transportin 3 as the causative gene for LGMD1F. PLoS One 8:e63536 (2013). WB ; Human . PubMed: 23667635