概述

  • 产品名称
  • 描述
    兔多克隆抗体to TNNI3K
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 756-805 (GRSHVAALRS RFELEYALNA RSYAALSQSA GQYSSQGLSL EEMKRSLQYT) of Human TNNI3K (NP_057062).

  • 阳性对照
    • HepG2 cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab86564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    May play a role in cardiac physiology.
  • 组织特异性
    Highly expressed in both adult and fetal heart.
  • 序列相似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 10 ANK repeats.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated.
  • 细胞定位
    Nucleus. Cytoplasm. Expressed at lower levels in the cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cardiac ankyrin repeat kinase antibody
    • cardiac muscle antibody
    • Cardiac troponin I-interacting kinase antibody
    • CARK antibody
    • CMD1FF antibody
    • CMD2A antibody
    • CMH7 antibody
    • cTnl antibody
    • MGC142099 antibody
    • MGC33828 antibody
    • RCM1 antibody
    • Serine/threonine protein kinase TNN13K antibody
    • Serine/threonine-protein kinase TNNI3K antibody
    • TNI3K_HUMAN antibody
    • TNNC1 antibody
    • TNNI3 interacting kinase antibody
    • TNNI3-interacting kinase antibody
    • Tnni3k antibody
    • Troponin 1 antibody
    see all

图片

  • Anti-TNNI3K antibody (ab86564) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 93 kDa

实验方案

文献

This product has been referenced in:
  • Theis JL  et al. TNNI3K mutation in familial syndrome of conduction system disease, atrial tachyarrhythmia and dilated cardiomyopathy. Hum Mol Genet N/A:N/A (2014). WB ; Mouse . Read more (PubMed: 24925317) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Dog Tissue lysate - whole (heart)
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Glen Pyle

Verified customer

提交于 Sep 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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