概述

  • 产品名称Anti-TNNI1抗体
    参阅全部 TNNI1 一抗
  • 描述
    兔多克隆抗体to TNNI1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide: KRPPLRRVRV SADAMLRALL GSKHKVSMDL RANLKSVKKE DTEKERPVEV, corresponding to amino acids 109-158 of Human TNNI1

  • 阳性对照
    • Jurkat cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab58783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

靶标

Anti-TNNI1 antibody 图像

  • Anti-TNNI1 antibody (ab58783) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk in PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 22 kDa
    Observed band size : 22 kDa
    Additional bands at : 63 kDa. We are unsure as to the identity of these extra bands.

Anti-TNNI1 antibody (ab58783)参考文献

ab58783 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"