Anti-TNNI1抗体[12F10] (ab8293)

概述

  • 产品名称Anti-TNNI1抗体[12F10]
    参阅全部 TNNI1 一抗
  • 描述
    小鼠单克隆抗体[12F10] to TNNI1
  • 特异性Detects only slow Troponin I from human skeletal muscle. Cross-reaction of this antibody with cardiac troponin I is less then 0.05%.
  • 经测试应用适用于: Sandwich ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) (Human).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS with 0.1% sodium azide, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号12F10
  • 骨髓瘤Sp2/0
  • 同种型IgG2b
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab8293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [7G2] to Troponin I fast + slow skeletal muscle (HRP) (ab24464). Use as Capture Antibody.
AP Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

靶标

  • 功能Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • 组织特异性Highest levels observed in human skeletal muscle (e.g. gastrocnemious muscle), differentiated cultures of primary human muscle cells and rhabdomyosarcoma cells cultured in low serum medium. Expressed in C2 muscle cell myoblasts and myotubes.
  • 序列相似性Belongs to the troponin I family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2700018B22Rik antibody
    • AI747285 antibody
    • DKFZp451O223 antibody
    • si:dz182n13.3 antibody
    • slow skeletal muscle antibody
    • slow-twitch isoform antibody
    • SSTNI antibody
    • TNN1 antibody
    • Tnni1 antibody
    • TNNI1_HUMAN antibody
    • Troponin I antibody
    • Troponin I slow skeletal muscle antibody
    • Troponin I slow twitch isoform antibody
    • troponin I type 1 (skeletal, slow) antibody
    • Troponin I, slow isoform antibody
    see all

Anti-TNNI1 antibody [12F10] 图像

  • All lanes : Anti-TNNI1 antibody [12F10] (ab8293) at 1 µg/ml

    Lane 1 : 40ug human muscle lysate (whole tissue)
    Lane 2 : 40ug human muscle lysate (whole tissue)
    Lane 3 : 40ug human muscle lysate (whole tissue)
    Lane 4 : 40ug human muscle lysate (whole tissue)
    Lane 5 : 40ug human muscle lysate (whole tissue)
    Lane 6 : 40ug human muscle lysate (whole tissue)
    Lane 7 : 40ug rat adipose lysate (whole tissue)

    Secondary
    HRP donkey anti-mouse HRP
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 22 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-TNNI1 antibody [12F10] (ab8293)参考文献

This product has been referenced in:
  • Zhou Y  et al. Pitx2 regulates myosin heavy chain isoform expression and multi-innervation in extraocular muscle. J Physiol 589:4601-14 (2011). Read more (PubMed: 21727215) »
  • Groshong JS  et al. Calpain activation impairs neuromuscular transmission in a mouse model of the slow-channel myasthenic syndrome. J Clin Invest 117:2903-12 (2007). WB ; Human . Read more (PubMed: 17853947) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (skeletal muscle)
Loading amount 30 µg
Specification skeletal muscle
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Aug 19 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Muscle biopsy material)
Loading amount 40 µg
Specification Muscle biopsy material
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5%
Username

Abcam user community

Verified customer

提交于 Oct 26 2006

Thank you for your enquiry. Unfortunately we have not determined the epitopes. However, the antibodies are forming a pair in ELISA. Recommended pair is 12F10 - 7G2 (capture - conjugate).

Unfortunately, we do not sell ab8294 conjugated to HRP. You will need to conjugate this antibody to HRP yourself in order to use it in a sandwich ELISA. ab9920 (human skeletal muscle Troponin I) should be suitable for use as a positive control (we can...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"