RabMAb

Anti-TNFSF9抗体[EPR1172Y] (ab68185)

概述

  • 产品名称Anti-TNFSF9抗体[EPR1172Y]
    参阅全部 TNFSF9 一抗
  • 描述
    兔单克隆抗体[EPR1172Y] to TNFSF9
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TNFSF9 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: 293T and Raji cell lysates. IP: HEK293 whole cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab68185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
IP 1/40 - 1/50.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages.
    • 组织特异性Expressed in brain, placenta, lung, skeletal muscle and kidney.
    • 序列相似性Belongs to the tumor necrosis factor family.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 4 1BB L antibody
      • 4 1BB ligand antibody
      • 4 1BBL antibody
      • 4-1BB ligand antibody
      • 4-1BBL antibody
      • Cd137l antibody
      • Cd157l antibody
      • Homolog of mouse 4 1BB L antibody
      • Homolog of mouse 4 1BBL antibody
      • ILA ligand (TNF related) antibody
      • Ly63l antibody
      • Receptor 4 1BB ligand antibody
      • TNFL9_HUMAN antibody
      • Tnfsf9 antibody
      • TNLG5A antibody
      • Tumor necrosis factor (ligand) superfamily member 9 antibody
      • Tumor necrosis factor ligand 5A antibody
      • Tumor necrosis factor ligand superfamily member 9 antibody
      • Tumor necrosis factor superfamily member 9 antibody
      see all

    Anti-TNFSF9 antibody [EPR1172Y] 图像

    • Anti-TNFSF9 antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + Raji whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 27 kDa
      Observed band size : 25,32 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Anti-TNFSF9 antibody [EPR1172Y] (ab68185) at 1/1000 dilution (purified) + HEK293 whole cell lysate at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

      Predicted band size : 27 kDa
      Observed band size : 25-32 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • ab68185 (purified) at 1/50 immunoprecipitating TNFSF9 in HEK293 whole cell lysate.

      Lane 1 (input): HEK293 whole cell lysate (10µg)

      Lane 2 (+): ab68185 + HEK293 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68185 in HEK293 whole cell lysate.

      For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-TNFSF9 antibody [EPR1172Y] (ab68185) at 1/2000 dilution (unpurified) + 293T cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 27 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    Anti-TNFSF9 antibody [EPR1172Y] (ab68185)参考文献

    ab68185 has not yet been referenced specifically in any publications.

    Product Wall


    For western blots and immunocytochemistry (ICC), we recommend HEK293T cell lysate for a positive control, and an antibody dilution of 1/500 -1/2000 for western blotting and 1/100 - 1/250 for ICC. We have the lysate as ab95494.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"