Anti-TNFAIP3抗体(ab74037)

概述

  • 产品名称Anti-TNFAIP3抗体
    参阅全部 TNFAIP3 一抗
  • 描述
    兔多克隆抗体to TNFAIP3
  • 特异性This antibody detects endogenous levels of total TNFAIP3 protein.
  • 经测试应用适用于: IHC-Fr, ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A synthesized peptide derived from the internal region of human TNFAIP3.

  • 阳性对照
    • HepG2 cells and extracts from RAW264.7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab74037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr
ELISA
WB
ICC/IF
  • 应用说明ELISA: 1/20000.
    ICC/IF: 1/500 - 1/1000.
    WB: 1/500 - 1/1000. Predicted molecular weight: 90 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. In vitro able to deubiquitinate both 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system.
    • 序列相似性Belongs to the peptidase C64 family.
      Contains 7 A20-type zinc fingers.
      Contains 1 OTU domain.
    • 结构域The A20-type zinc fingers mediate the ubiquitin ligase activity.
      The OTU domain mediates the deubiquitinase activity.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • A20 antibody
      • MGC104522 antibody
      • MGC138687 antibody
      • MGC138688 antibody
      • OTU domain containing protein 7C antibody
      • OTU domain-containing protein 7C antibody
      • OTUD7C antibody
      • Putative DNA binding protein A20 antibody
      • Putative DNA-binding protein A20 antibody
      • TNAP3_HUMAN antibody
      • TNF alpha-induced protein 3 antibody
      • TNFA1P2 antibody
      • TNFAIP 3 antibody
      • TNFAIP3 (A20) antibody
      • TNFAIP3 antibody
      • Tumor necrosis factor alpha induced protein 3 antibody
      • Tumor necrosis factor alpha-induced protein 3 antibody
      • Tumor necrosis factor induced protein 3 antibody
      • Tumor necrosis factor inducible protein A20 antibody
      • tumor necrosis factor, alpha-induced protein 3 antibody
      • Zinc finger protein A20 antibody
      see all

    Anti-TNFAIP3 antibody 图像

    • Immunofluorescence analysis of TNFAIP3 in HepG2 cells using ab74037 at a 1/500 dilution.
      Left image: untreated.
      Right image: treated with immunizing peptide.
    • All lanes : Anti-TNFAIP3 antibody (ab74037) at 1/500 dilution

      Lane 1 : Extracts from RAW264.7 cells
      Lane 2 : Extracts from RAW264.7 cells with immunizing peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 90 kDa
      Observed band size : 90 kDa
      Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
    • ab74037, at 1/50, staining TNFAIP3 in mouse tissue sections by immunohistochemistry (frozen sections).

      See Abreview

    Anti-TNFAIP3 antibody (ab74037)参考文献

    ab74037 has not yet been referenced specifically in any publications.

    Product Wall

    ab74037 to be tested in IHC-P: discount code ****
    ab109018 to be tested in ICC/IF: discount code *****

    Expiration date for both codes: DD MM YYYY

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Thymus)
    Specification Thymus
    Fixative Formaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 20°C
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    提交于 Aug 17 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"