概述

  • 产品名称Anti-TNFAIP1抗体
    参阅全部 TNFAIP1 一抗
  • 描述
    兔多克隆抗体to TNFAIP1
  • 特异性This antibody detects endogenous levels of total TNFAIP1 protein.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthesized peptide derived from an internal region of human TNFAIP1.

  • 阳性对照
    • Extracts from HUVEC cells.

性能

  • 形式Liquid
  • 存放说明Store at -20°C. Stable for 12 months at -20°C
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/5000.
    WB: 1/500 - 1/1000. Detects bands of approximately 34, 60 and 68 kDa (predicted molecular weight: 36 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(BACURD2) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.
    • 通路Protein modification; protein ubiquitination.
    • 序列相似性Belongs to the BACURD family.
      Contains 1 BTB (POZ) domain.
    • 翻译后修饰Phosphorylation at Ser-280 by CK2 facilitates the nucleus localization and increases interaction with PCNA.
    • 细胞定位Cytoplasm. Nucleus. Endosome. Colocalizes with RHOB in endosomes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • alpha-induced protein 1 antibody
      • BACD2_HUMAN antibody
      • BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 antibody
      • BTB/POZ domain-containing protein TNFAIP1 antibody
      • endothelial antibody
      • hBACURD2 antibody
      • Protein B12 antibody
      • tnfaip1 antibody
      • Tumor necrosis factor alpha induced protein 1 (endothelial) antibody
      • Tumor necrosis factor alpha induced protein 1 antibody
      • Tumor necrosis factor antibody
      see all

    Anti-TNFAIP1 antibody 图像

    • All lanes : Anti-TNFAIP1 antibody (ab72626) at 1/500 dilution

      Lane 1 : Extracts from HUVEC cells, plus PMA (125ng/ml, 30mins)
      Lane 2 : Extracts from HUVEC cells, plus serum (20%, 30mins)
      Lane 3 : Extracts from HUVEC cells, plus PMA (125ng/ml, 30mins) and serum (20%, 30mins) plus immunizing peptide

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 36 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 and 68 kDa. We are unsure as to the identity of these extra bands.

    Anti-TNFAIP1 antibody (ab72626)参考文献

    ab72626 has not yet been referenced specifically in any publications.

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