Anti-TNF Receptor II抗体[MR2-1] (ab8161)


  • 产品名称
    Anti-TNF Receptor II抗体[MR2-1]
    参阅全部 TNF Receptor II 一抗
  • 描述
    小鼠单克隆抗体[MR2-1] to TNF Receptor II
  • 宿主
  • 特异性
    The antibody reacts with the extra-cellular part of the TNF-R II and with the soluble receptor.
  • 经测试应用
    适用于: ICC/IF, WB, IP, Flow Cytmore details
  • 种属反应性
    与反应: Human, Cynomolgus monkey, Rhesus monkey
  • 免疫原

    soluble TNF-R75 from culture supernatant of cells transfected with the extracellular part of TNF-R75, purified by affinity chromatography on a TNF-coupled agarose column.



Our Abpromise guarantee covers the use of ab8161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
IP Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • 功能
    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
  • 序列相似性
    Contains 4 TNFR-Cys repeats.
  • 翻译后修饰
    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD120b antibody
    • p75 antibody
    • p75 TNF receptor antibody
    • p75TNFR antibody
    • p80 TNF alpha receptor antibody
    • p80 TNF-alpha receptor antibody
    • Soluble TNFR1B variant 1 antibody
    • TBP-2 antibody
    • TBPII antibody
    • TNF R II antibody
    • TNF R2 antibody
    • TNF R75 antibody
    • TNF-R2 antibody
    • TNF-RII antibody
    • TNFBR antibody
    • TNFR-II antibody
    • TNFR1B antibody
    • TNFR2 antibody
    • TNFR80 antibody
    • TNFRII antibody
    • Tnfrsf1b antibody
    • TNR1B_HUMAN antibody
    • Tumor necrosis factor beta receptor antibody
    • Tumor necrosis factor binding protein 2 antibody
    • Tumor necrosis factor receptor 2 antibody
    • Tumor necrosis factor receptor superfamily member 1B antibody
    • Tumor necrosis factor receptor type II antibody
    • Tumor necrosis factor-binding protein 2 antibody
    see all


  • ICC/IF image of ab8161 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • HDMECs were harvested with trypsin and washed three times with ice-cold PBS. Lysis buffer (50 mm Tris-HCl, pH 7.5, 10% of glycerol, 1% of Triton X-100, 300 mm NaCl, 150 mm KCl, 5 mm EDTA, 1 mm dithiothreitol, 0.5 mm Na3VO4, 10 mm NaF, 10 mm iodoacetamide, and protease inhibitors) or SDS gel buffer with ß-mercaptoethanol was then added, and the lysates were cleared of debris by centrifugation. 80 µg of total protein/sample was subjected to 4–15% gradient SDS-PAGE and electrophoretically transferred to polyvinylidene difluoride membranes. Protein blots on the membrane were identified by using ab8161 followed by horseradish peroxidase-conjugated anti-IgG secondary antibodies in blocking buffer. Images were obtained by chemiluminescence on x-ray film using ECL reagents.
  • Overlay histogram showing HL60 cells stained with ab8161 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8161, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.


This product has been referenced in:
  • Miettinen JA  et al. Tumor necrosis factor alpha promotes the expression of immunosuppressive proteins and enhances the cell growth in a human bone marrow-derived stem cell culture. Exp Cell Res 317:791-801 (2011). ICC/IF ; Human . Read more (PubMed: 21182837) »
  • Ding B  et al. FOXO3a regulates oxygen-responsive expression of tumor necrosis factor receptor 2 in human dermal microvascular endothelial cells. J Biol Chem 284:19331-9 (2009). WB ; Human . Read more (PubMed: 19473970) »

See all 4 Publications for this product


Thank you for contacting us. We may be able to custom make this antibody in an azide-free form for bulk orders of 10 or more vials. If you are interested in a custom bulk purchase, please contact my colleagues at and they can hel...

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Western blot
Human Cell lysate - whole cell (TF-1 cells)
Loading amount
30 µg
TF-1 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

提交于 Aug 01 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell lysate - whole cell (TF-1 cells)
Total protein in input
30 µg
TF-1 cells
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

提交于 Sep 18 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Flow Cytometry
Human Cell (Brain microvascular endothelial cells)
Brain microvascular endothelial cells

Abcam user community

Verified customer

提交于 Feb 02 2007

Thank you for your enquiry. We have just received the following information from the source of ab8161: The antibody to human CD120b, TNF-RII (p75/p80), clone MR2-1 were generated as described in the following article: Leeuwenberg, JFM et al; Slow r...

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I'm afraid that the precise AA seq that this antibody recognises has not been determined.

This antbody has not yet been tested on rat tissue

The agonistic properties of HM2007 are tested in a cytotoxicity assay with KYM-1 cells. In this cytotoxicity assay concentrations of 0.03 ; 0.3 and 3 microgram/ml antibody were used.