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Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab109322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 73 kDa (predicted molecular weight: 48 kDa).Can be blocked with TNF Receptor II peptide (ab192471).|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Antigen retrieval is recommended.|
|ICC||1/250 - 1/500.
For best results in ICC/IF please use 100% Methanol fixation.
|Flow Cyt||1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.|
ab109322 staining TNF Receptor II in SW480 cells. The cells were fixed with 100% methanol. In our hands PFA fixed cells did not produce good results with this antibody. Nuclear DNA was labelled in blue with DAPI.
Primary: ab109322, 1/1000 dilution
Sample: Jurkat whole cell lysate, 100 ug
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated, 1/1000 dilution
Capture antibody: 1:40 dilution (1µg in 1 mg lysate)
Blocking and diluting buffer: 5% NFDM/TBST