Anti-TNF Receptor II抗体(ab15563)

概述

  • 产品名称
    Anti-TNF Receptor II抗体
    参阅全部 TNF Receptor II 一抗
  • 描述
    兔多克隆抗体to TNF Receptor II
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Dog, Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (Mouse) (C terminal).

  • 阳性对照
    • Thymus

性能

应用

Our Abpromise guarantee covers the use of ab15563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 55 kDa.
IP Use a concentration of 2 µg/ml.
IHC-P 1/50.

靶标

  • 功能
    Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
  • 序列相似性
    Contains 4 TNFR-Cys repeats.
  • 翻译后修饰
    Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
    A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD120b antibody
    • p75 antibody
    • p75 TNF receptor antibody
    • p75TNFR antibody
    • p80 TNF alpha receptor antibody
    • p80 TNF-alpha receptor antibody
    • Soluble TNFR1B variant 1 antibody
    • TBP-2 antibody
    • TBPII antibody
    • TNF R II antibody
    • TNF R2 antibody
    • TNF R75 antibody
    • TNF-R2 antibody
    • TNF-RII antibody
    • TNFBR antibody
    • TNFR-II antibody
    • TNFR1B antibody
    • TNFR2 antibody
    • TNFR80 antibody
    • TNFRII antibody
    • Tnfrsf1b antibody
    • TNR1B_HUMAN antibody
    • Tumor necrosis factor beta receptor antibody
    • Tumor necrosis factor receptor 2 antibody
    • Tumor necrosis factor receptor superfamily member 1B antibody
    • Tumor necrosis factor receptor type II antibody
    • Tumor necrosis factor-binding protein 2 antibody
    see all

图片

  • ab15563 staining TNF Receptor II at 1/50 dilution in thymus by Immunohistochemistry (FFPE-sections).
  • Anti-TNF Receptor II antibody (ab15563) at 1/1000 dilution + whole cell lysate prepared from human platelets (treated with ADP for 30 minutes) at 20 µg

    Secondary
    HRP conjugated goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 55 kDa


    Exposure time : 2 minutes

    Image courtesy of Dr Mahesh Shivananjappa by Abreview.

    Blocked with 5% milk for 1 hour at 22°C.

    See Abreview

文献

This product has been referenced in:
  • Kim JE  et al. Status Epilepticus Induces Vasogenic Edema via Tumor Necrosis Factor-a/ Endothelin-1-Mediated Two Different Pathways. PLoS One 8:e74458 (2013). WB, ICC/IF ; Rat . Read more (PubMed: 24040253) »
  • Al-Zoubi M  et al. Creating a tumor-resistant microenvironment: cell-mediated delivery of TNFa completely prevents breast cancer tumor formation in vivo. Cell Cycle 12:480-90 (2013). Read more (PubMed: 23292149) »

See all 11 Publications for this product

客户评价及客户问答

Thank you for contacting us.

For ab15563, the protocol states for antigen retrieval "Boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Wash 3 times in buffer."

For ab1215...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (CD14+)
Loading amount
20 µg
Specification
CD14+
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.6% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Feb 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Monocytes from Apheresis)
Specification
Monocytes from Apheresis
Fixation
BD Cytofix/Cytoperm 20 min
Permeabilization
Yes - BD Cytofix/Cytoperm 20 min
Gating Strategy
Monocytes
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Dec 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Mahesh Shivananjappa

Verified customer

提交于 Nov 23 2011

Thank you for your inquiry. Unfortunately, we are not able to provide the blocking peptides for these antibodies. We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion...

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Thank you for contacting us. This antibody has been successfully tested in Dog and Human species in our lab, but has not been tested in Mouse. However, due to the high sequence homology between the protein’s immunogens, it is predicted to react...

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Thank you for your enquiry. I was not able to obtain a range for the sequence of this immunogen, but by looking closely at this antibody's data sheet in immunogen section, it states that the immunogen is a: "Synthetic peptide (the amino acid sequenc...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (TF-1 cells)
Total protein in input
30 µg
Specification
TF-1 cells
Immuno-precipitation step
Protein A/G
Username

Dr. Alison Taylor

Verified customer

提交于 Nov 08 2006

Thank you for your enquiry. The following antibodies fir your specifications (although we don't know if they have been tested on brain samples), please take a look at the online datasheets for additional information. Please contact us again if you have...

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Thank you for your email and I'm sorry to hear that you are experiencing difficulty with these two antibodies. For ab15563, it is suggested on the online datasheet to use thymus as a positive control. Also, TNF receptors are present on nearly all cell ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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