Anti-TNF beta抗体[AT15A3] (ab100844)


  • 产品名称Anti-TNF beta抗体[AT15A3]
    参阅全部 TNF beta 一抗
  • 描述
    小鼠单克隆抗体[AT15A3] to TNF beta
  • 经测试应用适用于: IHC-P, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 35-205 of Human TNF beta (NP_001153212)

  • 阳性对照
    • HeLa cells and Mouse Spleen lysate IHC-P: Human skin melanoma FFPE tissue sections.



Our Abpromise guarantee covers the use of ab100844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500. Predicted molecular weight: 22 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.


  • 功能Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo.
  • 疾病相关Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 细胞定位Secreted. Membrane. The homotrimer is secreted. The heterotrimer is membrane-associated.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LTalpha antibody
    • DIF antibody
    • LT alpha antibody
    • LT antibody
    • LT-alpha antibody
    • Lta antibody
    • Lymphotoxin alpha (TNF superfamily, member 1) antibody
    • Lymphotoxin alpha antibody
    • Lymphotoxin-alpha antibody
    • TNF B antibody
    • TNF superfamily member 1 antibody
    • TNF, lymphocyte-derived antibody
    • TNF-beta antibody
    • TNFB antibody
    • TNFB_HUMAN antibody
    • TNFbeta antibody
    • TNFSF1 antibody
    • TNLG1E antibody
    • Tumor necrosis factor beta antibody
    • tumor necrosis factor ligand 1E antibody
    • Tumor necrosis factor ligand superfamily member 1 antibody
    see all

Anti-TNF beta antibody [AT15A3] 图像

  • IHC image of ab100844 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab100844, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-TNF beta antibody [AT15A3] (ab100844) at 1/1000 dilution + Mouse Spleen Tissue Lysate at 35 µg

    goat anti-mouse conjugated to HRP
    developed using the ECL technique

    Predicted band size : 22 kDa
  • Immunofluorescence of Human HeLa cells stained with ab100844 at 1/500 with Texas Red (Red). Nucleus was stained by Hoechst 33342 (Blue).

Anti-TNF beta antibody [AT15A3] (ab100844)参考文献

ab100844 has not yet been referenced specifically in any publications.

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This antibody has been tested in indirect ELISA only; we are unsure about the sandwich ELISA pair. The epitope of this antibody has also not been mapped which is another obstacle for finding a matched pair based on...

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