Anti-TNF alpha抗体[V1q(H8)] (ab8097)


  • 产品名称
    Anti-TNF alpha抗体[V1q(H8)]
    参阅全部 TNF alpha 一抗
  • 描述
    大鼠单克隆抗体[V1q(H8)] to TNF alpha
  • 宿主
  • 特异性
    Anti-mouse TNF-a reacts with both natural and recombinant TNF-a and shows neutralizing activity.
  • 经测试应用
    适用于: Inhibition Assay, Neutralising, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Other Immunogen Type corresponding to Mouse TNF alpha. Cytotoxin purified from Concanavalin A induced T cell clone 29

  • 阳性对照
    • Monocytes/macrophages



Our Abpromise guarantee covers the use of ab8097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Inhibition Assay Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration. PubMed: 23300453

The antibody was functionally tested by neutralization of the cytopathic effect of cytotoxin in the L929 TNF bioassay. The biological activity of the antibody can be defined as the concentration of V1q required to neutralize 100 U/ml of TNF/cytotoxin.

Flow Cyt Use at an assay dependent concentration.

 Stains the extracellular domain. The CHO cells were fixed in PBA containing 0.2 % formaldehyde before staining.


  • 功能
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 疾病相关
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性
    Belongs to the tumor necrosis factor family.
  • 翻译后修饰
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all


This product has been referenced in:
  • Helk E  et al. TNFa-mediated liver destruction by kupffer cells and Ly6Chi monocytes during Entamoeba histolytica infection. PLoS Pathog 9:e1003096 (2013). Neutralising ; Mouse . Read more (PubMed: 23300453) »
  • Goren I  et al. Severely impaired insulin signaling in chronic wounds of diabetic ob/ob mice: a potential role of tumor necrosis factor-alpha. Am J Pathol 168:765-77 (2006). Read more (PubMed: 16507892) »

See all 3 Publications for this product


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