Anti-TNF alpha抗体[T1] (FITC) (ab65099)


  • 产品名称Anti-TNF alpha抗体[T1] (FITC)
    参阅全部 TNF alpha 一抗
  • 描述
    小鼠单克隆抗体[T1] to TNF alpha (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性This antibody reacts with the free soluble form (17 kDa) and the membrane (26 kDa) human TNF alpha. It does not react with receptor bound TNF alpha.
  • 经测试应用适用于: Neutralising, Flow Cyt, IP, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human TNF-alpha.

  • 常规说明This product should be filter sterilized and Azide removed by dialysis, before use in Biological assays.



Our Abpromise guarantee covers the use of ab65099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Neutralising Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB 1/10. Predicted molecular weight: 26 kDa.


  • 功能Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 疾病相关Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Anti-TNF alpha antibody [T1] (FITC) 图像

  • Overlay histogram showing HeLa cells stained with ab65099 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65099, 1µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells ). Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-TNF alpha antibody [T1] (FITC) (ab65099)参考文献

This product has been referenced in:
  • Gerspach J  et al. Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 50:243-50 (2000). Read more (PubMed: 10891890) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Sample Mouse Cell (uterus)
Permeabilization Yes - 0.1 % Triton-X-100 in PBS
Gating Strategy All cell populations were included
Specification uterus
Preparation Cell harvesting/tissue preparation method: Enzymatic digestion
Sample buffer: PBS
Fixation Paraformaldehyde

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提交于 May 21 2015