Anti-TNF alpha抗体[T1] (Biotin) (ab17357)


  • 产品名称Anti-TNF alpha抗体[T1] (Biotin)
    参阅全部 TNF alpha 一抗
  • 描述
    小鼠单克隆抗体[T1] to TNF alpha (Biotin)
  • 偶联物Biotin
  • 特异性The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha.
  • 经测试应用适用于: IP, Flow Cyt, WB, Neutralisingmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    This information is considered to be commercially sensitive.

  • 常规说明

    The antibody reacts with free soluble (17kDa) and membrane (26kDa) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII).



Our Abpromise guarantee covers the use of ab17357 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Detects a band of approximately 17, 26 kDa.
Neutralising Use at an assay dependent concentration.

For neutralization of biological activity in vitro dilutions have to be made according to the amounts of TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added.


  • 功能Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 疾病相关Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Anti-TNF alpha antibody [T1] (Biotin) (ab17357)参考文献

This product has been referenced in:
  • Gerspach J  et al. Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 50:243-50 (2000). Read more (PubMed: 10891890) »

See 1 Publication for this product

Product Wall

As for ab17442, it would also be a good choice, but personally if I had to chose, I would go for ab17357. The chances of have 2 antibodies that are against the exact same epitope region are slim, but it is something that you should be aware of.
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Although we have not directly tested ab17357 in a sandwich ELISA, the fact that has proven successful in IP and flow cytometry does bode well for it working in a sELISA and I think there is a really good chance that it will work.

The one issu...

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