Anti-TNF alpha抗体[MP6-XT22] - BSA and Azide free (ab34719)


  • 产品名称Anti-TNF alpha抗体[MP6-XT22] - BSA and Azide free
    参阅全部 TNF alpha 一抗
  • 描述
    大鼠单克隆抗体[MP6-XT22] to TNF alpha - BSA and Azide free
  • 经测试应用适用于: IHC-Fr, Functional Studies, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant full length protein corresponding to Mouse TNF alpha. Recombinant murine TNF alpha expressed in E. coli.
    Database link: P06804

  • 常规说明

    This clone (MP6-XT22) is reported to neutralise the effects of mouse TNF alpha.



Our Abpromise guarantee covers the use of ab34719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
ELISA Use a concentration of 1 - 10 µg/ml.
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl. Membrane permeabilisation is required for this application.

ab18407-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 疾病相关Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Anti-TNF alpha antibody [MP6-XT22] - BSA and Azide free (ab34719)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Coronal brain slices)
Specification Coronal brain slices

Miss. Ronit Shapira

Verified customer

提交于 Mar 23 2016

Thank you for your enquiry and interest.

As the datasheet indicates this product has been characterized on IHC-Fr (frozen section for immunohistochemistry) but NOT on IHC-P (formalin-fixed paraffin-embedded sections). The samples can be fixed...

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Thank you for your enquiry and your interest in our products. I can confirm that this antibody has been tested and characterized for in "in vitro" functional assay using cultured cells. I am very sorry to inform you that as the disclaimer states...

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