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Our Abpromise guarantee covers the use of ab9755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.
To detect rTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rTNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
|ELISA||Use a concentration of 0.5 µg/ml.
To detect rTNF-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rTNF-alpha.
|Neutralising||Use a concentration of 0.145 - 0.165 µg/ml.
To yield one-half maximal inhibition [ND50] of the biological activity of rTNF-alpha (2.0 ng/ml), a concentration of 0.145 - 0.165 µg/ml of this antibody is required
|ICC/IF||Use a concentration of 5 µg/ml.|