概述

  • 产品名称Anti-TNF alpha抗体
    参阅全部 TNF alpha 一抗
  • 描述
    兔多克隆抗体to TNF alpha
  • 特异性No reactivity is detected against human TNF-b (lymphotoxin). This antibody will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin.
  • 经测试应用适用于: ELISA, IHC-P, IHC-Fr, Neutralising, WB, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Human, Pig, Fish, Cynomolgus Monkey
    预测可用于: Dog, Monkey, Non Human Primates
  • 免疫原

    Recombinant full length protein corresponding to TNF alpha.
    Database link: P01375
    (Peptide available as ab140754)

  • 阳性对照
  • 常规说明Endotoxin content by LAL is <10 pg/ml.

性能

应用

Our Abpromise guarantee covers the use of ab6671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/200 - 1/1000.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/100 - 1/200.
Neutralising 1/200.
WB 1/500 - 1/2000. Can be blocked with Recombinant Human TNF alpha protein (ab140754).

Membrane Blocking is recommended with BSA not Milk for this product.

Suitable for use as a positive control for Western blot against recombinant TNFalpha produced in E.Coli. 

ICC/IF Use at an assay dependent concentration. PubMed: 19458984
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 疾病相关Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Anti-TNF alpha antibody 图像

  • ab6671 staining TNF alpha in Cynomolgus Monkey dendritic cells/macrophages from inlamed skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 20 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH6.0. Samples were incubated with primary antibody (1/100) for 30 minutes at room temperature. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.

    See Abreview

  • ab6671 staining TNF alpha in Guinea pig Lung and heart cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA in PBST) for 18 hours at 4°C. An Alexa Fluor® 555-conjugated Donkey anti-rabbit IgG (H+L) polyclonal (1/800) was used as the secondary antibody.

    See Abreview

  • Anti-TNF alpha antibody (ab6671) at 1/1000 dilution + Recombinant TNF-alpha protein

    Secondary
    Goat Anti-Rabbit Dylight® 649 Conjugate at 1/20000 dilution

    Additional bands at : 16 kDa (possible cleavage fragment).

    Western Blot analysis, labelling TNF-alpha with ab6671 at 1/1000. Blocking was with 1% BSA in TBS-T incubated for 30 minutes at room temperature. The Cleavage Fragment is related to the soluble isoform 77 - 223. 

  • ab6671 staining TNF alpha in Human testis & colon CA tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% H2O2 for 10 minutes at 25°C; antigen retrieval was by heat mediation using EDTA, pH9, 100C, 20 min. Samples were incubated with primary antibody (1/75) for 20 minutes at 25°C. An undiluted HRP polymer-conjugated Goat anti-mouse anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • ab6671 staining human artery tissue sections by IHC-P.  Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) prior to blocking with 1.5% serum for 10 minutes.  The primary antibody was diluted 1/100 and incubated with the sample for 24 hours at 4°C.  An HRP-conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • ab6671 staining TNF alpha from human colon by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 2 hours at 21°C. Alexa fluor® 680 goat polyclonal, diluted 1/5000, was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded fish tissue, staining TNF alpha with ab6671.

    Tissue was fixed with Bouin's fixative; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/600 in diluent) for 2 hours. An HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

Anti-TNF alpha antibody (ab6671)参考文献

This product has been referenced in:
  • Huang B  et al. Renal Tumor Necrosis Factor a Contributes to Hypertension in Dahl Salt-Sensitive Rats. Sci Rep 6:21960 (2016). IHC ; Rat . Read more (PubMed: 26916681) »
  • Chen J  et al. From the Cover: Zinc Deficiency Worsens and Supplementation Prevents High-Fat Diet Induced Vascular Inflammation, Oxidative Stress, and Pathological Remodeling. Toxicol Sci 153:124-36 (2016). Read more (PubMed: 27370414) »

See all 78 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human Synoviocyte fibroblast-like cell)
Gel Running Conditions Reduced Denaturing (9% SDS-PAGE)
Loading amount 20 µg
Treatment D-glucose 500 mg/dl for 24hrs
Specification Human Synoviocyte fibroblast-like cell
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 27 2016

Application Western blot
Sample Chicken Tissue lysate - whole (thymus)
Gel Running Conditions Reduced Denaturing (10-20% Tris-glycine)
Loading amount 20 µg
Specification thymus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Jenny Shoots

Verified customer

提交于 Dec 07 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 30 min
Permeabilization No
Specification brain
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 28 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung tissue, macrophages, monocytes)
Antigen retrieval step None
Permeabilization No
Specification Lung tissue, macrophages, monocytes
Blocking step Zytomed Systems HRP Polymer Kit Blocking Reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Hope-fixation
Username

Abcam user community

Verified customer

提交于 Oct 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer 1X
Permeabilization No
Specification kidney
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 14 2015

Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (liver)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 50 µg
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 29 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Sample Rabbit Tissue sections (iliac)
Specification iliac
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 04 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 26 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20 min
Sample Human Tissue sections (testis, colon CA)
Specification testis, colon CA
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 17 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Guinea pig Cell (Lung and Heart)
Specification Lung and Heart
Permeabilization No
Fixative Formalin
Username

Matthew Williams

Verified customer

提交于 Jan 30 2014

1-10 of 47 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"