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Recombinant full length protein corresponding to Mouse TNF alpha.
(Peptide available as
Our Abpromise guarantee covers the use of ab34674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/400 - 1/800.|
|WB||1/400 - 1/800. Detects a band of approximately 17 kDa (predicted molecular weight: 26 kDa).Can be blocked with Synthetic Mouse TNF alpha protein (ab151311). Detects TNF alpha from supernatants or lysates of 2 x 106 endotoxin stimulated mouse peripheral blood mononuclear cells (PBMC).|
|IHC-P||Use at an assay dependent concentration.|
|ELISA||1/1000 - 1/2000. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.|
|Neutralising||1/200. It is recommended to incubate the sample with the dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.|
Immunohistochemical analysis of frozen PFA perfusion-fixed murine dermal tissue sections, labelling TNF with ab34674 at a concentration of 1/200 incubated for 24 hours at 4°C in TBS. Permeablization was with Triton X-100. Blocking was with 5% serum incubated for 30 minutes at 22°C. The secondary was a polyclonal Donkey anti-rabbit Cy3® conjugate at 1/200.
ab34674 staining TNF alpha in mouse knee joint sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 10 minutes at room temperature followed by incubation with the primary antibody at a 1/200 dilution for 30 minutes. An undiluted HRP-conjugated human anti-rabbit monoclonal was used as secondary antibody.
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