Anti-TNF alpha抗体[52B83] (ab1793)

概述

  • 产品名称Anti-TNF alpha抗体[52B83]
    参阅全部 TNF alpha 一抗
  • 描述
    小鼠单克隆抗体[52B83] to TNF alpha
  • 特异性This antibody detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. It reacts with free soluble (17 kDa) and membrane (26 kDa) human TNF-alpha. It does not react with receptor-bound TNF-alpha. Non-specific background staining is observed in connective tissues.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, ICC/IF, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Guinea pig, Human, Chimpanzee, Zebrafish, Cynomolgus Monkey, Rhesus monkey, Apteronotus leptorhynchus
  • 免疫原

    Full length native protein (purified) (Human).

  • 阳性对照
  • 常规说明Dilution of 20-200 times is useful for IHC on paraffin embedded tissues and frozen sections; flow cytometry and Western blotting. Useful for staining classic paraffin embedded tissues without antigen retrieval.

性能

应用

Our Abpromise guarantee covers the use of ab1793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/10.

(methanol fixed cells)

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/50.

Fixed in acetone for 10 minutes

ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 17 kDa.

TNF-alpha is normally secreted as a homotrimer with a molecular mass of 52 kDa. Monomeric TNF-alpha  is 17.4 kDa

A reduced sample treatment and 15% SDS-Page was used.

IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • 疾病相关Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 翻译后修饰The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • APC1 antibody
    • APC1 protein antibody
    • Cachectin antibody
    • DIF antibody
    • Differentiation inducing factor antibody
    • Macrophage cytotoxic factor antibody
    • Tnf antibody
    • TNF superfamily, member 2 antibody
    • TNF, macrophage derived antibody
    • TNF, monocyte derived antibody
    • TNF-a antibody
    • TNF-alpha antibody
    • TNFA antibody
    • TNFA_HUMAN antibody
    • TNFSF2 antibody
    • Tumor necrosis factor alpha antibody
    • Tumor necrosis factor antibody
    • Tumor necrosis factor ligand superfamily member 2 antibody
    • Tumor Necrosis Factor, Membrane Form antibody
    • Tumor necrosis factor, soluble form antibody
    see all

Anti-TNF alpha antibody [52B83] 图像

  • ab1793 staining TNF alpha in mouse liver tissue sections, from mice intoxicated with carbon tetrachloride for 7 weeks, by IHC-P (Formaldehyde-fixed, Paraffin-embedded sections). Tissue samples were fixed with formaldehyde and blocked with 1% BSA for 30 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in 1% BSA) at 4°C for 12 hours. An undiluted HRP-conjugated secondary antibody was used.

    See Abreview

  • ab1793 staining TNFα in RAW 264.7 cells treated with (R,S)-rolipram (ab120029), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R,S)-rolipram, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120029 ((R,S)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • Overlay histogram showing RAW 264.7 cells stained with ab1793 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1793, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in RAW 264.7 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab1793 staining TNF alpha in Mouse duodenum and pancreatic cancer associated stroma sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained by DAPI.

    See Abreview

  • ab1793 staining TNF alpha in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with a MOM kit for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. An undiluted Biotin-conjugated Goat anti-mouse IgG polyclonal (MOM kit) was used as the secondary antibody.

    See Abreview

  • ab1793 staining TNFα in RAW 264.7 cells treated with (R)-(-)-rolipram (ab120031), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (R)-(-)-rolipram , as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120031 ((R)-(-)--rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • ab1793 staining TNFα in RAW 264.7 cells treated with (S)-(+)-rolipram (ab120030), by ICC/IF. Decrease in TNFα expression correlates with increased concentration of (S)-(+)-rolipram , as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120030 ((S)-(+)-rolipram) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1793(5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-TNF alpha antibody [52B83] (ab1793)参考文献

This product has been referenced in:
  • He D  et al. Enhanced M1/M2 macrophage ratio promotes orthodontic root resorption. J Dent Res 94:129-39 (2015). Read more (PubMed: 25344334) »
  • Inoue H  et al. Sonoporation-mediated transduction of siRNA ameliorated experimental arthritis using 3MHz pulsed ultrasound. Ultrasonics 54:874-81 (2014). Rat . Read more (PubMed: 24291002) »

See all 22 Publications for this product

Product Wall

Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Jan 22 2013

Thank you for your inquiry.

I am happy to confirm that ab1793 is tested and guaranteed for rat and WB and IHC-P.

I can confirm that we do not have any feedback about this antibody in perivascular adipose tissue yet. I therefore sugg...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for contacting us.

Unfortunately there is no information available about the epitope recognised by these two antibodies, as it hasn’t been mapped.

I am very sorry I couldn’t help you further. Please do not hesi...

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Thank you very much for your email.

My colleague is out of the office today but I will be happy to assist you with this issue.

I'm sending a free of charge vial of ab9739 on the order ***, which should arrive tomorrow. Please keep u...

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Thank you for your reply. Are you working in any species or applications other than WB on human samples? We have a large number of antibodies to TNF alpha that are guaranteed for that purpose. For example, our rabbit polyclonal antibodies ab6671 and ab...

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Thank you for your reply. Based on the protocol you provided, I would not expect the bands observed on your blot to be specific. I was able to locate an order that was shipped to *** last week for ab1793 and ab15563. Is this the correct order? If I am ...

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Thank you for contacting us. While TNF-alpha is known to form a homotrimer, the expected molecular weight for that band would be around 58 kDa. In order to help determine the nature of the bands you are observing, it would be helpful if you could pleas...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thats no problem at all. I'm glad you have found the information of help.

I am sorry that using the discount code online has not proved very straight forward. What you need to do is simply put in a normal order and add the discount code in th...

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