TMS1/ASC overexpression 293T裂解物(whole cell) (ab94128)
概述
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产品名称
TMS1/ASC overexpression 293T裂解物(whole cell)
参阅全部 TMS1/ASC 细胞裂解液 -
常规说明
ab94128 is a 293T cell transfected lysate in which Human TMS1 has been transiently over-expressed using a pCMV-TMS1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Tissue specificity: Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. Similarity: Contains 1 CARD domain. Contains 1 DAPIN domain. Domain: Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. PTM: Phosphorylated.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94128于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94128 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-TMS1/ASC antibody (ab89611) at 1/500 dilution
Lane 1 :TMS1/ASC overexpression 293T lysate (whole cell) (ab94128)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94128 尚未被引用在任何文献中。