TMRE-Mitochondrial膜Potential Assay试剂盒(ab113852)

概述

  • 产品名称TMRE-Mitochondrial膜Potential Assay试剂盒
    参阅全部 Mitochondrial Transmembrane Potential 试剂盒
  • 检测方法Fluorescent
  • 检测类型Cell-based (qualitative)
  • 产品概述

    Abcam's TMRE-Mitochondrial Membrane Potential Assay Kit (ab113852) is suitable for quantifying changes in mitochondrial membrane potential in live cells by flow cytometry, microplate spectrophotometry and fluorescent microscopy. Each assay kit contains sufficient materials for at least 200 measurements.

    ab113852 uses TMRE (tetramethylrhodamine, ethyl ester) to label active mitochondria. TMRE is a cell permeant, positively-charged, red-orange dye that readily accumulates in active mitochondria due to their relative negative charge. Depolarized or inactive mitochondria have decreased membrane potential and fail to sequester TMRE.

    FCCP (carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone) is a ionophore uncoupler of oxidative phosphorylation. Treating cells with FCCP eliminates mitochondrial membrane potential and TMRE staining. TMRE is suitable for the labeling of mitochondria in live cells and is not compatible with fixation.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Reagents

性能

  • 相关性Mitochondrial Transmembrane Potential is an important parameter of mitochondrial function used as an indicator of cell death. The collapse of the mitochondrial transmembrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
  • 别名
    • mitochondrial membrane potential

相关产品

应用

Our Abpromise guarantee covers the use of ab113852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

TMRE-Mitochondrial Membrane Potential Assay Kit 图像

  • A: HeLa cells (adherent) were cultured on coverslips and stained with ab113852 (200nM TMRE) for 20 minutes in media, washed briefly with PBS and immediately imaged. B: Jurkat cells (suspension) were stained and washed as above and then transferred to a slide and immobilized under a coverslip for imaging.
  • Chart showing mean fluorescent intensity +/- standard deviation from quadruplicate measurements of 400 nM TMRE stained Jurkat cells in a 96-well microplate +/- treatment with FCCP.

  • Flow cytometry histogram of Jurkat cells stained with ab113852 (100nM TMRE) with (blue) or without (red) treatment with 100µM FCCP.

实验方案

TMRE-Mitochondrial Membrane Potential Assay Kit (ab113852)参考文献

This product has been referenced in:
  • Jacque N  et al. Targeting glutaminolysis has antileukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition. Blood 126:1346-56 (2015). Read more (PubMed: 26186940) »
  • Maiti AK  et al. IL-4 Protects the Mitochondria Against TNFa and IFN? Induced Insult During Clearance of Infection with Citrobacter rodentium and Escherichia coli. Sci Rep 5:15434 (2015). Mouse . Read more (PubMed: 26481427) »

See all 9 Publications for this product

Product Wall

Thank you for contacting us. Our lab uses:

BD PureCoat™ Microplates
black/clear, polystyrene
96-well, amine
Cat#354717

The recommendation is for clear bottom, black wall microplate that (a) cells will grow on and (b...

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Thank you for your inquiry. I heard back from the lab regardingthese two distinct dyes that both measure mitochondrial membrane potential. TMRE stains mitochondria only when there is a membrane potential. JC-1 is slightly different in that it has dist...

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The samples should be read immediately after the 15-30 minute incubation with TMRE. Since the cells are live, as the health of the cell declined the signal from the dye would also diminish.







For another positive control, CCCP is suitable (5-50 μM CCCP for 30 to 60 minutes at 37ºC).

It all depends on the sample (embryos vs. explant vs. whole work). We have only worked with this dye using tissue culture cells (monolayer or suspension). If the samples are dissociated cells then TMRE labeling should work. Otherwise, we don’t kn...

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We have no experience in using TMRE to stain isolated mitochondria from any source – it is a tricky proposition as you would need intact/non-damaged mitochondria which retain membrane potential therefore we wouldn't recommend this.

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Thank you for contacting us. My colleague from our MitoSciences lab has provided the following recommendations.

The lack of signal may be due to working outside the peak excitation (549nm) and peak emission (575nm). Getting as close as possib...

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Thank you for contacting us.

I have received the following information from the lab:

TMRE can only be used with live cells—an active membrane potential is required for staining. This will be true of all membrane potential sen...

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Thank you for your inquiry.

Culture media is known to cause background using kit ab113852. For suspension cells, we recommend pelleting the cells, removing the culture media, resuspending in the same volume of 0.2% BSA in PBS, pelleting again...

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Thank you for contacting us.

Culture media is known to cause background using kit ab113852. For suspension cells, we recommend pelleting the cells, removing the culture media, resuspending in the same volume of 0.2% BSA in PBS, pelleting agai...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"