兔单克隆抗体[EPR3861] to TMPRSS2
Mouse, Rat, Human, Cynomolgus monkey, Rhesus monkey, Common marmoset
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TMPRSS2 aa 1-100.
Human prostate, Human small intestine and LNCaP cell lysates, Human prostatic adenocarcinoma
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these.
1/250 - 1/1000.
ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for IP.
Expressed strongly in small intestine. Also expressed in prostate, colon, stomach and salivary gland.
Belongs to the peptidase S1 family.
Contains 1 LDL-receptor class A domain. Contains 1 peptidase S1 domain. Contains 1 SRCR domain.
Cell membrane and Secreted. Activated by cleavage and secreted.
Information by UniProt
Western blot - TMPRSS2 antibody [EPR3861] (ab92323)
All lanes : Anti-TMPRSS2 antibody [EPR3861] (ab92323) at 1/1000 dilution Lane 1 : Human prostate lysate Lane 2 : Human small intestine lysate Lane 3 : LNCaP cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution Predicted band size: 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TMPRSS2 antibody [EPR3861] (ab92323)
ab92323 at 1/1000 dilution, staining TMPRSS2 in paraffin embedded Human prostatic adenocarcinoma
Flow Cytometry - Anti-TMPRSS2 antibody [EPR3861] (ab92323)
Overlay histogram showing LoVo cells stained with ab92323 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92323, 1µg/1x10
cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10
cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"