antigen sequence, corresponding to internal sequence amino acids 288-398 of Human TMPPE.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/100 - 1/250. Predicted molecular weight: 49 kDa.
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-TMPPE antibody 图像
Immunocytochemistry/ Immunofluorescence - Anti-TMPPE antibody (ab121904)
Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab121904 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-TMPPE antibody (ab121904)
All lanes : Anti-TMPPE antibody (ab121904) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver
Lane 5 : Human tonsil
Predicted band size : 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMPPE antibody (ab121904)
ab121904 at 1/125 dilution staining TMPPE in Paraffin-embedded Human Colon tissue by Immunohistochemistry.
Anti-TMPPE antibody (ab121904)参考文献
has not yet been referenced specifically in any publications.
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