Anti-TMIE抗体(ab103028)

概述

  • 产品名称
    Anti-TMIE抗体
  • 描述
    兔多克隆抗体to TMIE
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 78-108 of Human TMIE

  • 阳性对照
    • Mouse liver nuclear lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/500. Predicted molecular weight: 17 kDa.

靶标

  • 相关性
    The TMIE gene encodes a transmembrane inner ear protein. TMIE is required for normal postnatal maturation of sensory hair cells in the cochlea, including correct development of stereocilia bundles, in mice. It is one of multiple genes responsible for recessive non-syndromic deafness (DFNB). This is also known as autosomal recessive nonsyndromic hearing loss (ARNSHL), the most common form of congenitally acquired inherited hearing impairment.
  • 细胞定位
    Membrane; Single-pass type I membrane protein
  • 数据库链接
  • 别名
    • deafness autosomal recessive 6 antibody
    • DFNB6 antibody
    • transmembrane inner ear antibody
    • transmembrane inner ear expressed protein antibody
    • transmembrane inner ear protein antibody
    see all

图片

  • ab103028 staining TMIE in mouse COS-7 cells transfected with TMIE-cherry plasmid by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 4% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/400) for 1 hour at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Anti-TMIE antibody (ab103028) at 1/100 dilution + Mouse liver tissue lysate at 12.5 µg

    Predicted band size : 17 kDa

文献

ab103028 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (COS-7 cells)
Specification
COS-7 cells
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde
Username

Dr. Angela Ballesteros Morcillo

Verified customer

提交于 May 02 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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