antigen: CRQRYCRPRD LLQRYDSKPI VDLIGAMETQ SEPSELELDD VVITNPHIEA ILENEDWIED ASGLMSHCIA ILKICHTLTE KLVAMTMGSG AKMKTSASVS, corresponding to amino acids 27-126 of Human TMEM98 according to Q9Y2Y6.
Human rectum tissue. TMEM98 over-expression lysate from HEK293.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Belongs to the TMEM98 family.
Information by UniProt
Protein TADA1 antibody
Transmembrane protein 98 antibody
Immunocytochemistry/ Immunofluorescence - Anti-TMEM98 antibody (ab122654)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122654 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM98 antibody (ab122654)
ab122654 at 1/20 dilution staining TMEM98 in paraffin-embedded Human rectum tissue by Immunohistochemistry.
Western blot - Anti-TMEM98 antibody (ab122654)
All lanes : Anti-TMEM98 antibody (ab122654) at 1/250 dilution Lane 1 : Negative control (vector only transfected HEK293T lysate) Lane 2 : TMEM98 over-expression lysate (co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells Developed using the ECL technique.
This product has been referenced in:
et al. Inhibition of IL-8-mediated endothelial adhesion, VSMCs proliferation and migration by siRNA-TMEM98 suggests TMEM98's emerging role in atherosclerosis. Oncotarget 8:88043-88058 (2017).
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