重组Anti-EMC4抗体[EPR15081] (ab184162)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15081] to EMC4
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-EMC4抗体[EPR15081]
参阅全部 EMC4 一抗 -
描述
兔单克隆抗体[EPR15081] to EMC4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 293, SH-SY5Y, U87-MG and HepG2 whole cell lysate (ab7900); Human brain tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15081 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184162于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
May mediate anti-apoptotic activity. -
组织特异性
Isoform 1 is expressed in brain and heart. Isoform 2 is expressed in heart. -
序列相似性
Belongs to the TMEM85 family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 51234 Human
- Entrez Gene: 68032 Mouse
- Entrez Gene: 296049 Rat
- SwissProt: Q5J8M3 Human
- SwissProt: Q9CZX9 Mouse
- Unigene: 250905 Human
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别名
- Cell proliferation-inducing gene 17 protein antibody
- EMC4 antibody
- ER membrane protein complex subunit 4 antibody
see all
图片
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All lanes : Anti-EMC4 antibody [EPR15081] (ab184162) at 1/5000 dilution
Lane 1 : 293 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
All lanes : Anti-EMC4 antibody [EPR15081] (ab184162) at 1/20000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling EMC4 with ab184162 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab184162 被引用在 3 文献中.
- Coukos R et al. An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens. Elife 10:N/A (2021). PubMed: 34414886
- Lin DL et al. The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection. Cell Rep 27:1666-1674.e4 (2019). PubMed: 31067454
- Tang X et al. EMC3 coordinates surfactant protein and lipid homeostasis required for respiration. J Clin Invest 127:4314-4325 (2017). PubMed: 29083321