Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
Synthetic peptide corresponding to a region within the internal sequence amino acids 216-265 (RLEKESARLG AHLDRCVIAG LCLLTLGGVI LSCLLMMSMW KGELYRRNRF) of human TMEM74 (NP_694560).
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Immunogen affinity purified
- 纯化说明Purified by peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
- 相关性TMEM74 plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction. It is expressed in heart, lung and placenta.
- 细胞定位Lysosome membrane; Multi pass membrane protein. Cytoplasmic vesicle, autophagosome membrane; Multi pass membrane protein.
- Transmembrane protein 74 antibody
Anti-TMEM74 antibody 图像
Western blot - TMEM74 antibody (ab87239)
Anti-TMEM74 antibody (ab87239) at 1 µg/ml + human fetal heart lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 33 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.
Anti-TMEM74 antibody (ab87239)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"