Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
Synthetic peptide corresponding to a region within the internal sequence amino acids 216-265 (RLEKESARLG AHLDRCVIAG LCLLTLGGVI LSCLLMMSMW KGELYRRNRF) of human TMEM74 (NP_694560).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 33 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
TMEM74 plays an essential role in autophagy. TMEM74-induced autophagy may involve PI3K signal transduction. It is expressed in heart, lung and placenta.
Lysosome membrane; Multi pass membrane protein. Cytoplasmic vesicle, autophagosome membrane; Multi pass membrane protein.
- Transmembrane protein 74 antibody
Western blot - TMEM74 antibody (ab87239)
Anti-TMEM74 antibody (ab87239) at 1 µg/ml + human fetal heart lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
33 kDaObserved band size:
37 kDa (why is the actual band size different from the predicted?
)Additional bands at:
20 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 12%
has not yet been referenced specifically in any publications.
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