Anti-TMEM66抗体(ab107694)

概述

  • 产品名称Anti-TMEM66抗体
    参阅全部 TMEM66 一抗
  • 描述
    兔多克隆抗体to TMEM66
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 36-66 of Human TMEM66 (NP_057211.4).

  • 阳性对照
    • 293 cell line and Mouse cerebellum tissue lysates. IF/ICC: RAW 246.7 cell line.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab107694 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab107694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 37 kDa.

靶标

Anti-TMEM66 antibody 图像

  • ICC/IF image of ab107694 stained RAW 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107694, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-TMEM66 antibody (ab107694) at 1/100 dilution + 293 cell line lysates at 35 µg

    Predicted band size : 37 kDa
  • Anti-TMEM66 antibody (ab107694) at 1/100 dilution + Mouse cerebellum tissue lysates at 35 µg

    Predicted band size : 37 kDa

Anti-TMEM66 antibody (ab107694)参考文献

ab107694 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"