antigen sequence: FHTHFYDRLQ DAGSRWPELY LYSRADEVVL ARDIERMVEA RLARRVLARS VDFVSSAHVS HLRDYPTYYT SLCVDFMRNC VRC, corresponding to amino acids 195-277 of Human TMEM53.
- TMEM53 over-expression HEK293T lysate; Human colon tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
Belongs to the TMEM53 family.
Information by UniProt
- FLJ22353 antibody
- NET4 antibody
- RP4-678E16.2 antibody
Immunocytochemistry/ Immunofluorescence - Anti-TMEM53 antibody (ab121648)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in golgi apparatus and focal adhesions. Recommended concentration of ab121648 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-TMEM53 antibody (ab121648)
All lanes : Anti-TMEM53 antibody (ab121648) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : TMEM53 over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
10-20% SDS PAGE
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM53 antibody (ab121648)
ab121648 at 1/40 dilution staining TMEM53 in Paraffin-embedded Human colon tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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